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Dictionary » D » Degraded DegradedDegraded 1. Reduced in rank, character, or reputation; debased; sunken; low; base. The Netherlands . . . Were reduced practically to a very degraded condition. (Motley) 2. (Science: biology) Having the typical characters or organs in a partially developed condition, or lacking certain parts. Some families of plants are degraded dicotyledons. (Dana) Having steps; said of a cross each of whose extremities finishes in steps growing larger as they leave the center; termed also on degrees. ![]()
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Results from our forumDurability of steedman's wax embedded samples for FISH... later I tried to make another FISH but it didn't work. Does somebody know, if maybe my embedded samples are too old - maybe the ribosomes are degraded?
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Electroporation... that they are being incubated too long. What might be the cause of this? The only thing I can think might be the problem is her antibiotic being degraded/the wrong concentration? (ampicillin) Any other ideas would be gratefully received
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Re: Recombinant DNA not degraded inside transformed cells, why?I found some explanation here: (last paragraph) http://www.tru.ca/faculty/dnelson/courses/biol335/335notes/3recdna/1-restriction/recDNA1.html Thanks.
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Re: Recombinant DNA not degraded inside transformed cells, why?Here's one from PLOS Genetics today, right on time. Zhang G, Wang W, Deng A, Sun Z, Zhang Y, et al. (2012) A Mimicking-of-DNA-Methylation-Patterns Pipeline for Overcoming the Restriction Barrier of Bacteria. PLoS Genet 8(9): e1002987. doi:10.1371/journal.pgen.1002987 http://www.plosgenetics.org/arti...
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Re: Recombinant DNA not degraded inside transformed cells, why?Escape from restriction endonucleases is a valid point. That is totally a chance dependent phenomenon. Thanks.
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