Degradation

i would say you may have degradation. A quick thing you can do is run a small quantity of it on a gel, and see if it is all the same size. If you get a smear, you had degradation.

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... Is abundant in milk Is present mainly as a surface receptor on B-cells Is present with unusual frequency in myelomas Is resistant to proteolytic degradation 5) Select the ONE answer that is CORRECT. IgA in seromucus secretions: Is dimeric Cannot bind to neutrophils Has no J-chain Has no secretory ...

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Protein degradation also depends on the particular protein you're working with. Some proteins seem to be more sensible than others, though I don't know reasons (I mean, I can imagine some, but I never actually looked it up).

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Dear, thnx for reply.actually as i tld i've precipitated protein by acetone precipitation. In this precipitation method we get pellet which is protein and after centrifugation v have to collect the pellet and dissolve it into resuspension buffer.my problem is that my protein pellet is not getting fu...

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can anybody tell that after how much time protein at room temperature will degrade? secondly, i've a doubt that after acetone precipitation i'm getting pellet which is not getting fully dissolved in resuspension buffer.what can be the reason and how can i dissolve the pellet of protein without any m...

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