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Defined medium

Defined medium

(Science: cell culture) cell culture medium in which all components are known. In practice this means that the serum (that is normally added to culture medium for animal cells) is replaced by insulin, transferrin and possibly specific growth factors such as platelet-derived growth factor.


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Re:

Sorry for brining up an old thread but... Organisms with a defined, heritable body plan. That's perfect for ruling out prokaryotes and ... http://ijs.sgmjournals.org/content/57/6/1318/F1.medium.gif They too have a defined shape (a spiral ball with flagella on ...

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by BlahBlahBlah
Thu Jun 27, 2013 1:46 pm
 
Forum: Microbiology
Topic: Multicellular prokaryote
Replies: 24
Views: 17568

EC50 depending on cell number?

... cells. I find out that I I have a receptor activation EC50 at 1 uM in a medium voume of 50 ul. If I now repeat the same test with 5000 cells per ... at 1uM. Or better always at 1uM. This because the EC50 is ultimately defined by affinity of 1 molecule compound to one receptor molecule. Now ...

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by qforthemass
Tue Aug 14, 2012 9:40 pm
 
Forum: Cell Biology
Topic: EC50 depending on cell number?
Replies: 1
Views: 2456

Re: something really confused

... fertilizer numbers. We've no idea what's in the media mix -- if it is a defined medium, then in principle the fertilizer numbers could be calculated without doing additional ...

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by jonmoulton
Mon Feb 20, 2012 4:35 pm
 
Forum: General Discussion
Topic: something really confused
Replies: 2
Views: 976

Life is nonmaterial phenomenon

... is the foundational atom of modern biology, the “gene” also remains undefined. Likewise “species” is the foundation of evolutionary biology, but ... computer software is also nonphysical although it requires a physical medium (disk) for storage. Similarly biosoftware also requires a physical ...

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by PAWahid
Fri Jan 20, 2012 10:37 am
 
Forum: Molecular Biology
Topic: Life is nonmaterial phenomenon
Replies: 4
Views: 4724

The best medium for the growth of those two microorganisms

bacteria: http://www.teknova.com/HALOBACTERIUM-HALOBIUM-DEFINED-MEDIUM-p/h5011.htm fungus: https://mycotopia.net/forums/front-page-news/19812-amanita-cultivation-grape-juice.html

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by JackBean
Sat Oct 22, 2011 12:07 pm
 
Forum: Cell Biology
Topic: The best medium for the growth of those two microorganisms
Replies: 3
Views: 1427
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