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Dictionary » D » Decant

Decant

Decant

to pour off gently the upper clear portion of a fluid, leaving the sediment in the vessel.

Origin: Mediev. L. Decantho, fr. De-_ canthus, the beak of a jug, fr. G. Kanthos, corner of the eye


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plasmid prcipitation

1. precitate in 100% ethanol fow at least 1 hour (or overnight) at -20. 2. spin the sample (pellet the DNA) 3. decant supernatant. 4. wash with 70% etoh.... {spin the sample (pellet the DNA)} 5. decant/remove supernatant 6. allow DNA pellet to air-dry 7. resuspend in PBS

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by LilKim
Thu Feb 22, 2007 6:10 am
 
Forum: Molecular Biology
Topic: plasmid prcipitation
Replies: 6
Views: 959


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