Dictionary » D » Decant



to pour off gently the upper clear portion of a fluid, leaving the sediment in the vessel.

Origin: Mediev. L. Decantho, fr. De-_ canthus, the beak of a jug, fr. G. Kanthos, corner of the eye

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Chelex 100

... some time, and I have found that the best extraction results are as follows: 1)spin down a sample to a pellet in a 1.5ml microcentrifuge tube. 2)Decant supernatant 3)Add 100 micro liters of 5% chelex 100 (shake first to get the solution to mix, and pipette quickly into your sample before the ...

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by workforfood
Thu Nov 03, 2011 5:17 pm
Forum: Molecular Biology
Topic: Chelex 100
Replies: 6
Views: 13292

plasmid prcipitation

1. precitate in 100% ethanol fow at least 1 hour (or overnight) at -20. 2. spin the sample (pellet the DNA) 3. decant supernatant. 4. wash with 70% etoh.... {spin the sample (pellet the DNA)} 5. decant/remove supernatant 6. allow DNA pellet to air-dry 7. resuspend in PBS

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by LilKim
Thu Feb 22, 2007 6:10 am
Forum: Molecular Biology
Topic: plasmid prcipitation
Replies: 6
Views: 3314

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