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Deae-dextran

Deae-dextran

(Science: chemical) Diethylaminoethyldextran. Used as a support for ion-exchange chromatography.

chemical name: dextran, 2-(diethylamino)ethyl ether


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Interpretation & Troubleshooting on SDS/Native PAGE

Dear All, I'm currently doing purification on the glutamate decarboxylase. After running both DEAE and gel filtration purification, I did both SDS and Native PAGE to verify my results. For SDS PAGE, there were 3 faint bands found and all of it are very near to each other. ...

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by schroddycat
Sat Dec 15, 2012 4:12 pm
 
Forum: Molecular Biology
Topic: Interpretation & Troubleshooting on SDS/Native PAGE
Replies: 1
Views: 1444

Serious cloning issues - gel extractions?

... uncut vector, but by this point I am fairly certain it is a problem with gel extraction using these kits. The next step is to try using DEAE membrane and salt elution. Time is also an issue, as I only have 6 months left in my PhD project, and I have been grappling with this problem since ...

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by TaintedCherub
Thu Oct 15, 2009 11:27 am
 
Forum: Molecular Biology
Topic: Serious cloning issues - gel extractions?
Replies: 23
Views: 18617

HELP: osteoclasts purification

... think Sephadex could do much for resolving whole cells). There are modified Sephadex resins for ion exchange, but those would have names like DEAE-Sephadex, not Sephadex G10. So, I guess I'm skeptical that the method uses the G10 resin to separate cells, but I could be wrong.

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by blcr11
Mon Jun 18, 2007 8:22 am
 
Forum: Cell Biology
Topic: HELP: osteoclasts purification
Replies: 1
Views: 2165

The Fiber Disease

... of both porphyric and normal rats was obtained. Incubation conditions, the effect of salts and chelating agents, the chromatographic behavior in DEAE-cellulose and Sephadex G-100 columns, were comparatively studied with both enzymatic preparations. The effect of sodium diethyldithiocarbamate, ...

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by London
Thu Dec 28, 2006 6:33 am
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 4990824


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