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Dam methylase

Dam methylase

(Science: enzyme) An enzyme responsible for the methylation of adenine residues in specific sequences.

Synonym: deoxyadenosine methylase.


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Replacing the genetic material

In humans, methylation occurs on the cytosine nucleotide. It is methylated by DNA methylases (DNMT1 - which is usually the maintenance DNA methylase as it has been found to be attached to the polymerase complex and prefers the hemi-methylated DNA strand, in which ...

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by kolean
Sun Jul 04, 2010 7:53 pm
 
Forum: Genetics
Topic: Replacing the genetic material
Replies: 9
Views: 5279

EcoRI

... that modify nucleotides in the recognition site on the host DNA. E.coli makes the restriction enzyme EcoRI and the methylating enzyme EcoRI methylase. The methylase modifies EcoRI sites in the bacteria’s own genome to prevent it from being digested.

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by kikkid
Fri Oct 05, 2007 7:30 pm
 
Forum: Genetics
Topic: EcoRI
Replies: 2
Views: 2580

The Fiber Disease

... such as that of bacteriophages (host DNA is specifically modified at these sites). type I restriction endonucleases occur as a complex with the methylase and a polypeptide that binds to the recognition site on dNA. They are often not very specific and cut at a remote site. type II restriction ...

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by al
Sun Dec 24, 2006 4:08 am
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 5571866

The Fiber Disease

... domain)" Function Code:9.10 - Metabolism of Cofactors and Vitamins, Porphyrin and chlorophyll metabolism " /product="precorrin-3 methylase" "Function Code:13.08 - Other, Adhesion " /product="adhesion protein" "Function Code:12.02 - Cell Processes, ...

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by Nadas Moksha
Sun Aug 27, 2006 2:40 pm
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 5571866

BclI digestion

... your plasmid is from SCS110 cells which cuts only in the presence of dam methylase. Thanks for the quick reply, but I'm afraid I still don't get it. What should I do ...

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by pjansen
Wed Feb 01, 2006 7:37 pm
 
Forum: Molecular Biology
Topic: BclI digestion
Replies: 10
Views: 4997
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