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Lynn, contemporary Australian molecular biologist.

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I need some actual DNA sequences for a computer program.

I have been using random sequences up until now. Its easy enough to pick out a gene looking for start/stop codons (or Shine-Dalgarno sequences) but the 'gene' it identifies is not a multiple of 3 bases. The program then assumes a deletion/insertion mutation (or equivalent) resulting ...

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by Awol
Fri Mar 30, 2012 1:10 pm
Forum: Molecular Biology
Topic: I need some actual DNA sequences for a computer program.
Replies: 7
Views: 3612

DNA coding strand

... strand. Find the -35 concensus region. Find the -10 TA rich region show where transcription begins and write the mRNA transcript. Find the Shine Dalgarno sequence Identify the stop codon Identify the ATG start and translate the peptide. Add an insertion of one A immediately following the ATG ...

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by bomb4268
Thu Mar 11, 2010 3:15 pm
Forum: Genetics
Topic: DNA coding strand
Replies: 2
Views: 4265

cloning and activity problem

... DH5alpha and induce with 0.5 mM IPTG there is no difference on SDS gel between Host and Clones. Cloned genes has own promotor region and Shine-dalgarno sequence. I have tried different IPTG concentration, different time of induction, different media but still I see same SDS gel profile. (Cloned ...

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by denrul
Sun Oct 25, 2009 1:22 pm
Forum: Molecular Biology
Topic: cloning and activity problem
Replies: 5
Views: 2266

When are parts of prokaryotic operons seperated?

generally there is one Shine-Dalgarno(watch the spelling) sequence for every gene

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by MrMistery
Fri Nov 07, 2008 1:30 am
Forum: Genetics
Topic: When are parts of prokaryotic operons seperated?
Replies: 5
Views: 5891

Shine dalgarno sequence

Think of it as a way of controlling how often a baseball pitching machine throws a ball. If there is a Shine-Dalgarno gear in place, the machine throws 100 balls a minute. If you take out the gear, it only throws 10 balls a minute. If each ball represents 1 polypeptide chain, ...

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by blcr11
Thu Mar 13, 2008 1:53 pm
Forum: Molecular Biology
Topic: Shine dalgarno sequence
Replies: 6
Views: 4346
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