D enzyme

Hi guys, I'm a new guy in molecular biology. I plan to express a scfv in S2 cell using the plasmid pMT/bip/V5-His A (with enzyme site BglII and XbaI). Because there's a Bip signal sequence ahead of the enzyme site BglII, so I CAN NOT add Kozak sequence in the N-Term of my scfv gene, ...

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... because so many telomeres in so many places have been lost. However, in people with cancer, the gene that activates the synthesis of the enzyme telomerase (a huge protein complex that adds the sequence TTAGGG to telomeres to repair them), which causes tumor cells to not have a Hayflick ...

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Hi everybody I've been looking for a while now, and haven't come across this anywhere. Is glutamine synthetase (enzyme synthesizing glutamine from glutamate and ammonia) present in heterotrophs, e.g animals? This is quite a big deal, because it is the primary pahtway of ammonia ...

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Hi all, I have a question and I would like you to help me figure it out. Sometimes we read about solution contents for example the enzyme solution used in protoplast isolation, well in the paper its written like this 20 mM MES 0.4 M mannitol 20 mM KCl 10 mM CaCl2 0.1% BSA 1.5% Cellulase ...

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... are pretty faint. How does your sample look now on gel exclusion chromatography? What was source of your protein? I guess it wasn't native enzyme, because then you could hardly have only three bands (unless you've loaded very low amount of proteins, with which would correspond the faintness ...

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