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D enzyme

D enzyme --> 4-alpha-d-glucanotransferase

dextrin transglycosylase or glycosyltransferase;a 4-glycosyltransferase converting maltodextrins into amylose and glucose by transferring parts of 1,4-glucan chains to new 4-positions on glucose or other 1,4-glucans.

Synonym: amylomaltase, d enzyme, dextrin glycosyltransferase, dextrin transglycosylase, disproportionating enzyme.


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Souther blot and restriction mapping

... for Southern hybridisation. The probe was a 2.4 kb XhoI fragment. Using the southern data, determine the restriction map for genomic DNA. Data: Enzyme Approximate Band Sizes E ------------- 6.0 ----- 1.8 X ------------- 2.4 B ------------ >10 ----- 3.6 XE ------------ 1.7 ----- 0.7 XB ------------ ...

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by Mistersnuffle
Sat Oct 04, 2014 11:17 am
 
Forum: Genetics
Topic: Souther blot and restriction mapping
Replies: 0
Views: 26

principle of error-prone PCR???

Thanks so much for your answer. But how exactly the size of Mn ions affect the Taq enzyme? Did they changes the fourth-level structure of the Taq enzyme, such as combine with the regulatory subunit to control the activity, or something else. I mean, it is obviously ...

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by feixiaorou
Wed Oct 01, 2014 3:51 am
 
Forum: Molecular Biology
Topic: principle of error-prone PCR???
Replies: 2
Views: 325

Gene Upregulation/Increased Expression in HMG-CoA Reductase

... will result in an increase in the protein product that a gene is coding for. Meaning that if you increased expression of a gene that codes for an enzyme, it would result in upregulation of that enzyme. ^What's wrong with my thinking on this? If you want specifics, the discussion was on low serum ...

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by Upperninety8
Tue Sep 30, 2014 3:21 am
 
Forum: Genetics
Topic: Gene Upregulation/Increased Expression in HMG-CoA Reductase
Replies: 0
Views: 51

principle of error-prone PCR???

... by a classmate that Mn2+ ions is the key of error-prone PCR. I checked on the internet and I got nothing. I always thought the low-fidelity PCR enzyme is the reason leading to the error matches of basicpair. I am now confused, which one is correct? If I am wrong, please be kind explain the principle ...

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by feixiaorou
Tue Sep 30, 2014 1:23 am
 
Forum: Molecular Biology
Topic: principle of error-prone PCR???
Replies: 2
Views: 325

The effect of low temperature on Ascorbic Acid

And why do you think it's without enzyme?

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by JackBean
Wed Aug 20, 2014 6:36 pm
 
Forum: Molecular Biology
Topic: The effect of low temperature on Ascorbic Acid
Replies: 4
Views: 845
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