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D cells


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mRNA vs. cell reporter assay

If the cells autofluoresce, this could confound interpretation of the GTP signal. if you are looking for downregulation of transcription, it takes a while for preexisting GPR to degrade but the mRNA signal is likely to disappear ...

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by jonmoulton
Mon Sep 08, 2014 5:27 pm
 
Forum: Cell Biology
Topic: mRNA vs. cell reporter assay
Replies: 2
Views: 381

Re: Chromatin

... cell. For a moment, before cytokinesis, the dividing cell contains 92 chromosomes, but when the cell membrane seals up each of the daughter cells is back to 46 chromosomes (the 23 pairs, each pair consisting of a paternal and a maternal chromosome). OK, so I promised to ignore it but I can't ...

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by jonmoulton
Thu Sep 04, 2014 3:04 pm
 
Forum: Genetics
Topic: Chromatin
Replies: 2
Views: 186

Stem Cells

I think you can localize stem cells in lower organisms such as C. elegance by flurochrome labeled antibodies specific to specific proteins of stem cell.

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by josem
Wed Sep 03, 2014 9:32 pm
 
Forum: Genetics
Topic: Stem Cells
Replies: 5
Views: 4544

Re: Chromosome Differences

The DNA is never connected into a long single molecule in human cells. The DNA remains distributed into the 23 pairs of chromosomes (plus the mitochondrial chromosome), each single DNA molecules complexed with proteins. Each chromosome is replicated and ...

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by jonmoulton
Wed Sep 03, 2014 3:06 pm
 
Forum: Genetics
Topic: Chromosome Differences
Replies: 5
Views: 288

use of a bacterial protease inhibitor cocktail with mammalia

... a mammalian cell lysate without these latter 2 inhibitors? The specific cell types which we are using are: HUVEC (human umbilical vein endothelial cells) and HT-29 adenocarcinoma cell line. Dr Robert Weinberg Dept of Biology, MIT email: rweinber@mit.edu fax: 617-977-0902

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by rpwdoc
Tue Aug 26, 2014 10:08 pm
 
Forum: Cell Biology
Topic: use of a bacterial protease inhibitor cocktail with mammalia
Replies: 0
Views: 93
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