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DNA sequence

Dna sequence

the order of nucleotide bases in the dna molecule;

a succession of nucleotides in a dna molecule or strand; a succession of any number of nucleotides greater than four is liable to be called a sequence. With regard to its biological function, which may depend on context, a sequence may be sense or anti-sense, and either coding or noncoding. Dna sequences can also contain "junk DNA".


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CRISPR/Cas technology

... is in LD with your desired mutation, it can be bred out in 1-2 generations and even still, the off targets are primarily in intergenic noncoding sequence space ad exist in a homozygous state. Most people don't see much off-targeting. It took less than a year to make the mouse, generate the data ...

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by animodel123
Wed Mar 18, 2015 6:39 am
 
Forum: Genetics
Topic: CRISPR/Cas technology
Replies: 1
Views: 201

Re: How to read this tricky electropherogram?

Do you know if this is genomic DNA or cDNA? If not, you should BLAST against both the RNA sequences and the genomic DNA sequence.

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by jonmoulton
Mon Feb 23, 2015 3:50 pm
 
Forum: Molecular Biology
Topic: How to read this tricky electropherogram?
Replies: 1
Views: 515

How to read this tricky electropherogram?

I am doing an exercise to find the sequence of this electropherogram and confirming the results in NCBI Blast site. I wrote the sequence on the graph to the best of my ability of ~100 bp segments and searched them in Blast... nothing came ...

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by kabtq9s
Sat Feb 21, 2015 3:23 am
 
Forum: Molecular Biology
Topic: How to read this tricky electropherogram?
Replies: 1
Views: 515

Re: Use of cDNA when studying splicing

... be transcribe into proteins. because the proteins are translated from mature mRNA, scientist would associate the protein of interest to the mRNA sequence and therefore reverse transcribing to the cDNA sequence to know the specific gene for that particular protein.

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by DrGavinSee
Mon Feb 16, 2015 7:06 am
 
Forum: Molecular Biology
Topic: Use of cDNA when studying splicing
Replies: 3
Views: 1390

Protein sequence

Hello, I want to know what is the name of protein with sequence "cgt tct ata gat acg cga tga cgg tat acc atc cgc aaa gt" or how can i identify protein from sequence. Thanks a lot for answer becouse I tried couple ways (for example BLAST, uniprot, ...

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by morsagh1
Fri Feb 06, 2015 8:33 pm
 
Forum: Genetics
Topic: Protein sequence
Replies: 0
Views: 232
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