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DEAE-cellulose

Definition

noun

(1) A positively charged resin used in ion-exchange chromatography for protein purification and separation.

(2) A cellulose derivative that functions as anion exchanger in chromatography and in other industrial applications.


Supplement

DEAE-cellulose is actually an abbreviated form of diethylaminoethyl-cellulose.


Variant: DEAE-C

Synonym: cellulose, 2-(diethylamino)ethyl ether


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Results from our forum


Interpretation & Troubleshooting on SDS/Native PAGE

Dear All, I'm currently doing purification on the glutamate decarboxylase. After running both DEAE and gel filtration purification, I did both SDS and Native PAGE to verify my results. For SDS PAGE, there were 3 faint bands found and all of it are very near to each other. ...

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by schroddycat
Sat Dec 15, 2012 4:12 pm
 
Forum: Molecular Biology
Topic: Interpretation & Troubleshooting on SDS/Native PAGE
Replies: 1
Views: 1877

Serious cloning issues - gel extractions?

... uncut vector, but by this point I am fairly certain it is a problem with gel extraction using these kits. The next step is to try using DEAE membrane and salt elution. Time is also an issue, as I only have 6 months left in my PhD project, and I have been grappling with this problem since ...

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by TaintedCherub
Thu Oct 15, 2009 11:27 am
 
Forum: Molecular Biology
Topic: Serious cloning issues - gel extractions?
Replies: 23
Views: 20756

HELP: osteoclasts purification

... think Sephadex could do much for resolving whole cells). There are modified Sephadex resins for ion exchange, but those would have names like DEAE-Sephadex, not Sephadex G10. So, I guess I'm skeptical that the method uses the G10 resin to separate cells, but I could be wrong.

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by blcr11
Mon Jun 18, 2007 8:22 am
 
Forum: Cell Biology
Topic: HELP: osteoclasts purification
Replies: 1
Views: 2344


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