DEAE-C

Dear All, I'm currently doing purification on the glutamate decarboxylase. After running both DEAE and gel filtration purification, I did both SDS and Native PAGE to verify my results. For SDS PAGE, there were 3 faint bands found and all of it are very near to each other. ...

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... uncut vector, but by this point I am fairly certain it is a problem with gel extraction using these kits. The next step is to try using DEAE membrane and salt elution. Time is also an issue, as I only have 6 months left in my PhD project, and I have been grappling with this problem since ...

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... think Sephadex could do much for resolving whole cells). There are modified Sephadex resins for ion exchange, but those would have names like DEAE-Sephadex, not Sephadex G10. So, I guess I'm skeptical that the method uses the G10 resin to separate cells, but I could be wrong.

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... of both porphyric and normal rats was obtained. Incubation conditions, the effect of salts and chelating agents, the chromatographic behavior in DEAE-cellulose and Sephadex G-100 columns, were comparatively studied with both enzymatic preparations. The effect of sodium diethyldithiocarbamate, ...

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