Dictionary » D » D-alanyl-l-alanine endopeptidase

D-alanyl-l-alanine endopeptidase

D-alanyl-l-alanine endopeptidase

(Science: enzyme) acts on peptidoglycan

registry number: EC 3.4.99.-

Synonym: d-ala-l-ala endopeptidase


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Re: Gluten-free and Vaccines

... factor contributing to the resistance of the 33-mer gliadin peptide to gastrointestinal breakdown. Therefore, we hypothesized that a prolyl endopeptidase could catalyze breakdown of this peptide, thereby diminishing its toxic effects. Preliminary in vitro studies with short gliadin peptides ...

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by tmerezko
Tue Jun 22, 2010 6:08 pm
 
Forum: General Discussion
Topic: Gluten-free and Vaccines
Replies: 7
Views: 17823

6X His Tag vector construction assistance needed

... terminus before the 6xHis tag. However, you may have a problem if you try to remove your 6xHis tag later, depending on whether you use an exo- or endopeptidase. The endopeptidase should work fine, but if you use an exopeptidase and there is a "stop residue" for it before your 6xHis tag, ...

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by snowcapk
Fri May 02, 2008 3:51 am
 
Forum: Molecular Biology
Topic: 6X His Tag vector construction assistance needed
Replies: 2
Views: 6465

Re: Fractination of SA into Cytosol., Cell Wall, Cytoplasmic

... no need to load more than 1-5 ul of the cytoplasmic fracton as they are usually concentrated LYSOSTAPHIN (Sigma cat no L-7386), a glycyl-glycine endopeptidase from Staph. staphylolyticus that cleaves pentaglycine bridges crosslinking the peptidoglycan in the cell wall - store at -20C. DNase (Roche) ...

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by Ewa
Wed Jul 04, 2007 6:23 pm
 
Forum: Microbiology
Topic: Fractination of SA into Cytosol., Cell Wall, Cytoplasmic
Replies: 3
Views: 5110

Exopeptidase

... - Any of a group of enzymes that catalyze the hydrolysis of single amino acids from the end of a polypeptide chain. (from an online dictionary) An endopeptidase catalyses the hydrolysis of single amino acids in the middle of a polypeptide chain. Doesnt a dipeptidase just catalyze the hydrolysis ...

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by Chris4
Mon May 23, 2005 4:22 pm
 
Forum: Molecular Biology
Topic: Exopeptidase
Replies: 2
Views: 3558


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