D-alanyl-l-alanine endopeptidase

... terminus before the 6xHis tag. However, you may have a problem if you try to remove your 6xHis tag later, depending on whether you use an exo- or endopeptidase. The endopeptidase should work fine, but if you use an exopeptidase and there is a "stop residue" for it before your 6xHis tag, ...

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... no need to load more than 1-5 ul of the cytoplasmic fracton as they are usually concentrated LYSOSTAPHIN (Sigma cat no L-7386), a glycyl-glycine endopeptidase from Staph. staphylolyticus that cleaves pentaglycine bridges crosslinking the peptidoglycan in the cell wall - store at -20C. DNase (Roche) ...

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... - Any of a group of enzymes that catalyze the hydrolysis of single amino acids from the end of a polypeptide chain. (from an online dictionary) An endopeptidase catalyses the hydrolysis of single amino acids in the middle of a polypeptide chain. Doesnt a dipeptidase just catalyze the hydrolysis ...

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