Cytometry

... of counting extracted plant mitochondria? I would need en exact number of the mitochondria that I extracted. I don't have conditions for Flow cytometry and I'm also not interested in proteins to use Bradford assay. I'm thinking about plase contrast microscopy. Could anyone give me a protocol ...

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to assess the % of gamma/delta T cells in flow cytometry , i use 10µl instead of 20µl/500000 cells of monoclonal antibodies as recommend by the manufacturing. Now i am aking if that will infuence my results. thanks for helps

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... however I have been told that since neutrophils and monocytes are both highly granulated, they are difficult to be distinguished just by Flow Cytometry. Hence, sorting by Luminex which uses specific antibodies attached to microspheres could be a good tool to separate them.

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What type of FACS you are talking about? BD, for example, has tutorials and manuals online, just go to their flow cytometry page and pick your machine. The support pages are at: http://www.bdbiosciences.com/immunocytometry_systems/support/training/ They also have good customer support ...

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I have recently been trying to count E. coli using a Petroff-Hauser counting chamber. I wanted to count cells straight out of my glycerol stocks, but I am having difficulties. The E. coli looks strange directly after the glycerol stock is thawed. My Procedure: Thaw the E. coli cell bank (glycerol st...

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