Dictionary » C » Cytometry



The counting of cells, especially blood cells, using a cytometer or haemocytometer.

flow cytometry, a method of measuring fluorescence from stained cells that are in suspension and flowing through a narrow orifice, usually in combination with one or two lasers to activate the dyes; used to measure cell size, number, viability, and nucleic acid content with the aid of acridine orange, kastens fluorescent feulgen stain, ethidium bromide, trypan blue, and other selected staining reagents.

Synonym: flow cytophotometry.

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Measuring ROS with DCFH probe

... really appreciate your opinions on the folllowing situation. I'm trying to measure ROS in HUVEC cells with the fluorescent probe DCFH by flow cytometry. I apply oxidant and antioxidant treatments, and what I get is that the cells with an oxidant treatment show less fluorescence than the control ...

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by rem
Wed Oct 30, 2013 6:49 pm
Forum: Molecular Biology
Topic: Measuring ROS with DCFH probe
Replies: 1
Views: 1351

Fixing CFSE labeled cells

Hi all, I want to label cells with CFSE, culture them, collect cells at different time points and analyse them all at once by flow cytometry. I was planning to fix the cells with 75% ethanol after collecting them. Is this possible / reliable? Or does fixation / storage affect the signal? ...

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by BiolMC
Tue Jun 11, 2013 12:34 pm
Forum: Cell Biology
Topic: Fixing CFSE labeled cells
Replies: 0
Views: 769

Stem Cells

... stems cells, there are cell surface markers (cluster of differentiation; CD) that can also be used to track down these cells, either with flow cytometry or other monoclonal antibody/fluorescence-based assays. The problem is that the stem cells are usually very rare and thus difficult to detect, ...

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by biohazard
Tue May 14, 2013 6:56 am
Forum: Molecular Biology
Topic: Stem Cells
Replies: 4
Views: 2586

Microparticles and flow cytometry

I have a question regarding flow cytometry too!!! Please heeelp. I have a lot of questions based on the cytometry pictures, where they ask to calculate percentage of dead cells, or of CD45R etc. Does anyone know how to guide me through the ...

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by simina
Thu Feb 28, 2013 5:43 pm
Forum: Molecular Biology
Topic: Microparticles and flow cytometry
Replies: 5
Views: 2291

Animal-Derived Laboratory Reagents

... about animal-derived laboratory reagents and products. I’m working in a strictly in-vitro lab that focuses mainly on western blots and flow cytometry. I was wondering the cost to animal life associated with these reagents. These include: FBS (fetal bovine serum) & BSA (bovine serum albumen): ...

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by southtowns18
Thu Dec 06, 2012 6:13 pm
Forum: General Discussion
Topic: Animal-Derived Laboratory Reagents
Replies: 0
Views: 1181
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