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Cytometry

Cytometry

The counting of cells, especially blood cells, using a cytometer or haemocytometer.

flow cytometry, a method of measuring fluorescence from stained cells that are in suspension and flowing through a narrow orifice, usually in combination with one or two lasers to activate the dyes; used to measure cell size, number, viability, and nucleic acid content with the aid of acridine orange, kastens fluorescent feulgen stain, ethidium bromide, trypan blue, and other selected staining reagents.

Synonym: flow cytophotometry.


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Why cells dies in flow cytometry analysis??

Why are all my cells in flow cytometry analysis dead??

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by w19910411
Wed Mar 11, 2015 8:46 am
 
Forum: Cell Biology
Topic: Why cells dies in flow cytometry analysis??
Replies: 1
Views: 415

Flow cytometry

Hi there, So I have carried out flow three times now to measure fluorescence (each time one sample per what I was looking at i.e no triplicates or anything)...and each of the three times the exp was carried out the same way. Would it be OK to combine the fluorescence of three exp, get average/standa...

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by biology_06er
Mon Oct 13, 2014 11:37 pm
 
Forum: Molecular Biology
Topic: Flow cytometry
Replies: 0
Views: 482

Measuring ROS with DCFH probe

... really appreciate your opinions on the folllowing situation. I'm trying to measure ROS in HUVEC cells with the fluorescent probe DCFH by flow cytometry. I apply oxidant and antioxidant treatments, and what I get is that the cells with an oxidant treatment show less fluorescence than the control ...

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by rem
Wed Oct 30, 2013 6:49 pm
 
Forum: Molecular Biology
Topic: Measuring ROS with DCFH probe
Replies: 1
Views: 1846

Fixing CFSE labeled cells

Hi all, I want to label cells with CFSE, culture them, collect cells at different time points and analyse them all at once by flow cytometry. I was planning to fix the cells with 75% ethanol after collecting them. Is this possible / reliable? Or does fixation / storage affect the signal? ...

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by BiolMC
Tue Jun 11, 2013 12:34 pm
 
Forum: Cell Biology
Topic: Fixing CFSE labeled cells
Replies: 0
Views: 1025

Stem Cells

... stems cells, there are cell surface markers (cluster of differentiation; CD) that can also be used to track down these cells, either with flow cytometry or other monoclonal antibody/fluorescence-based assays. The problem is that the stem cells are usually very rare and thus difficult to detect, ...

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by biohazard
Tue May 14, 2013 6:56 am
 
Forum: Molecular Biology
Topic: Stem Cells
Replies: 4
Views: 3155
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