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Cysteine s-conjugate n-acetyltransferase

Cysteine s-conjugate n-acetyltransferase

(Science: enzyme) catalyses n-acetylation of l-cysteine thioesters to form mercapturic acids; important in drug detoxication

registry number: EC 2.3.1.80

Synonym: cscn acetyltransferase


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Re: Measure of Cysteine concentration

You will measure, dilute and measure until you get an absorbance between 1 and 2 (in the Beer's law range). As Jack wrote, you need a molar extinction coefficient. Your path length is likely 1cm for a garden-variety spectrometer. Solve Beer's law for the concentration, then plug in your values for t...

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Measure of Cysteine concentration

... 3 with less starting sample. Measure the absorbance at 324 nm. Note the absorbance (A324 of reaction) 4. Calculation the protein concentration and cysteine concentration of the samples.

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