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Cysteine s-conjugate n-acetyltransferase

Cysteine s-conjugate n-acetyltransferase

(Science: enzyme) catalyses n-acetylation of l-cysteine thioesters to form mercapturic acids; important in drug detoxication

registry number: EC 2.3.1.80

Synonym: cscn acetyltransferase


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Re: Measure of Cysteine concentration

You will measure, dilute and measure until you get an absorbance between 1 and 2 (in the Beer's law range). As Jack wrote, you need a molar extinction coefficient. Your path length is likely 1cm for a garden-variety spectrometer. Solve Beer's law for the concentration, then plug in your values for t...

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by jonmoulton
Thu Nov 15, 2012 4:23 pm
 
Forum: Molecular Biology
Topic: Measure of Cysteine concentration
Replies: 2
Views: 1417

Measure of Cysteine concentration

... 3 with less starting sample. Measure the absorbance at 324 nm. Note the absorbance (A324 of reaction) 4. Calculation the protein concentration and cysteine concentration of the samples.

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by scholesmu
Wed Nov 14, 2012 5:11 pm
 
Forum: Molecular Biology
Topic: Measure of Cysteine concentration
Replies: 2
Views: 1417

Help with unkown

... Red + Vouges - Proskauer Test - Catalase + Oxidase - nitrate + starch hydrolysis - no amylase casein hydrolysis - Lipase + tryptophanase + urea - cysteine desulfurase hydrogen sulfide production. Citrate - phenylalanase - litmus milk - Lysine decarboxylase - I think it's Shigella flexneri, but ...

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by bruserboy1
Thu Apr 05, 2012 3:48 pm
 
Forum: Microbiology
Topic: Help with unkown
Replies: 0
Views: 1096

Re: how to memorize the amino acid

... Glycine is of course an exception. Proline as well, since it is an imino acid. Alanine is the "basic" amino acid. Now, serine and cysteine are hydroxy- and thiol derivatives. Valine and leucine are dimethyl and isopropyl derivatives. Isoleucine is not iso- for no reason, so you ...

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by JackBean
Thu Feb 09, 2012 9:52 am
 
Forum: General Discussion
Topic: how to memorize the amino acid
Replies: 4
Views: 5185

BCYE agar plates and legionella culture

... -Ketoglutarate stimulates organism growth. Ferric Pyrophosphate supplies iron. Agar is the solidifying agent. BCYE Agar is supplemented with L-Cysteine, an essential amino acid incorporated to satisfy specific nutritional requirements of Legionella spp. Selective agents can be added if necessary. ...

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by LedBulb900Lumen
Thu Jan 05, 2012 2:37 pm
 
Forum: Microbiology
Topic: BCYE agar plates and legionella culture
Replies: 3
Views: 3006
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