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Cycle

Cycle

a round or succession of observable phenomena, recurring usually at regular intervals and in the same sequence.

Origin: gr. Kyklos = circle The unit of frequency; one hertz has a periodic interval of one second.


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amplification in no RT control

Hi, what is 20 cycles later? In my qPCR everything after cycle 40-ish was just noise. Can you see a band on a gel after a standard PCR?

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by Tricho
Thu Mar 26, 2015 10:27 pm
 
Forum: General Discussion
Topic: amplification in no RT control
Replies: 1
Views: 160

Ligase reaction problem

... an image you can see below. Here, I want to ask whether it is enough base lenght for ligase reaction via thermostable ligase in 63 C (Protocol: 5 cycle at 95 C-30 s and 63 C-4 min) I think I take cross reaction because of this dimer but I am not sure. Is it possible to discard this short undesired ...

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by Molbio34
Thu Feb 26, 2015 8:27 am
 
Forum: Molecular Biology
Topic: Ligase reaction problem
Replies: 0
Views: 347

Biological Immortality and Immortalised Cell lines

... are several methods can be used to generate immortalized cell lines. One method is to introduce a viral gene that partially deregulates the cell cycle, such as EBV, SV40 T antigens and HPV-16 E6/7 gene. Artificial expression of key proteins required for immortality is another approach to induce ...

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by 2444256703
Thu Jan 22, 2015 8:58 am
 
Forum: Cell Biology
Topic: Biological Immortality and Immortalised Cell lines
Replies: 0
Views: 817

Re: The evolution of facilitation and semelparity

... meant r selected. This given semelparous species we shall call A. Now A, being an annual species, has a very short life history. Its entire life cycle will be completed in a year or less, and thus we might presume that during this time, it has to go about its business of development, maturation ...

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by Youngji
Sun Jan 11, 2015 11:55 am
 
Forum: Evolution
Topic: The evolution of facilitation and semelparity
Replies: 3
Views: 1038

Re: Please read! About life history strategies!

... help elucidate the ecology of this given species we shall call A. Now A, being an annual species, has a very short life history. Its entire life cycle will be completed in a year or less, and thus we might presume that during this time, it has to go about its business of development, maturation ...

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by Youngji
Sun Jan 11, 2015 11:30 am
 
Forum: Ecology
Topic: Please read! About life history strategies!
Replies: 2
Views: 1054
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