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Cuvette

Cuvette

(Science: apparatus) a transparent or translucent box-shaped container with precisely-measured dimensions for holding liquid samples to be put into a spectrophotometer.


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Re: Lonza MycoAlert test

... but like all products has a few pitfalls. Questions: 1.) are you using a multifunction plate reader with a luminescence setting or a dedicated cuvette luminometer? 2.) Are you using MycoAlert or MycoAlert Plus? Some multifunction plate readers have hopelessly weak luminescence detectors and ...

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by buzzbomb711
Fri Jul 25, 2014 6:51 am
 
Forum: General Discussion
Topic: Lonza MycoAlert test
Replies: 1
Views: 1405

Re: Device for ratiometric fluorescence measurement

... bands, you would build a 2-band fluorescence colorimeter. You'd need two emission filters and one or two excitation filters, arranged around your cuvette so that the excitation and emission light paths intersect at a 90 degree angle in the sample. Of course, you'd need an excitation source and ...

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by jonmoulton
Mon Mar 04, 2013 4:58 pm
 
Forum: General Discussion
Topic: Device for ratiometric fluorescence measurement
Replies: 1
Views: 1389

Can particle size affect spectrophotometry?

Hey guys, I'm not sure where else to put this...doesn't seem to fit anywhere else. But if you have a lipid sample in a cuvette, I know that changing the concentration will affect the absorbance readings. But what about particle size? For example, I was working with two different lipids ...

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by keetner
Thu Nov 29, 2012 3:24 am
 
Forum: General Discussion
Topic: Can particle size affect spectrophotometry?
Replies: 1
Views: 1108

Measure of Cysteine concentration

i have no idea how the route of the calculation. 1. 2.6 ml UV-assay buffer add to cuvette 2. 50 ul sample add to curvette and measure absorbance 324 nm (A324 nm of sample) 3. 200 ul DTDP solution add to curvette. If the absorbance is greater than 1, repeat 2 and ...

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by scholesmu
Wed Nov 14, 2012 5:11 pm
 
Forum: Molecular Biology
Topic: Measure of Cysteine concentration
Replies: 2
Views: 1479

solutions containing different concentrations of oxaloacetat

... 100 μL OAA malate: 900 μL water + 100 μL malate NADH: 900 μL water + 100 μL NADH we set the wavelength of the spectrophotometer to 340 nm. used a cuvette with 1 mL water as black, and them measured the absorbance of each of the dilutions of OAA, malate, or NADH, mixing each thoroughly before taking ...

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by ladwer
Sun Oct 07, 2012 2:38 am
 
Forum: General Discussion
Topic: solutions containing different concentrations of oxaloacetat
Replies: 1
Views: 1735
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