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Cuvette

Cuvette

(Science: apparatus) a transparent or translucent box-shaped container with precisely-measured dimensions for holding liquid samples to be put into a spectrophotometer.


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Re: Device for ratiometric fluorescence measurement

... bands, you would build a 2-band fluorescence colorimeter. You'd need two emission filters and one or two excitation filters, arranged around your cuvette so that the excitation and emission light paths intersect at a 90 degree angle in the sample. Of course, you'd need an excitation source and ...

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by jonmoulton
Mon Mar 04, 2013 4:58 pm
 
Forum: General Discussion
Topic: Device for ratiometric fluorescence measurement
Replies: 1
Views: 973

Can particle size affect spectrophotometry?

Hey guys, I'm not sure where else to put this...doesn't seem to fit anywhere else. But if you have a lipid sample in a cuvette, I know that changing the concentration will affect the absorbance readings. But what about particle size? For example, I was working with two different lipids ...

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by keetner
Thu Nov 29, 2012 3:24 am
 
Forum: General Discussion
Topic: Can particle size affect spectrophotometry?
Replies: 1
Views: 871

Measure of Cysteine concentration

i have no idea how the route of the calculation. 1. 2.6 ml UV-assay buffer add to cuvette 2. 50 ul sample add to curvette and measure absorbance 324 nm (A324 nm of sample) 3. 200 ul DTDP solution add to curvette. If the absorbance is greater than 1, repeat 2 and ...

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by scholesmu
Wed Nov 14, 2012 5:11 pm
 
Forum: Molecular Biology
Topic: Measure of Cysteine concentration
Replies: 2
Views: 1084

solutions containing different concentrations of oxaloacetat

... 100 μL OAA malate: 900 μL water + 100 μL malate NADH: 900 μL water + 100 μL NADH we set the wavelength of the spectrophotometer to 340 nm. used a cuvette with 1 mL water as black, and them measured the absorbance of each of the dilutions of OAA, malate, or NADH, mixing each thoroughly before taking ...

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by ladwer
Sun Oct 07, 2012 2:38 am
 
Forum: General Discussion
Topic: solutions containing different concentrations of oxaloacetat
Replies: 1
Views: 1337

Re: glass cuvette cleaning

... water, not the other way around) followed by extensive repeated rinsing in distilled deionized water would be more than needed to clean the cuvette. The rinsing is critical -- get all the water off the cuvette between rinses and I'd do at least five rinses. Using smaller volumes of rinse ...

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by jonmoulton
Tue Mar 06, 2012 4:50 pm
 
Forum: General Discussion
Topic: glass cuvette cleaning
Replies: 2
Views: 2379
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