Cuvette

In an experiment it is found that the optical density of a solution of compound X was 0.7 when measured at 400nm. in a 1cm glass cuvette. The solution had been prepared by dissolving 35mg of X in 50 ml of water. What is the molar extinction coefficient of X at 400nm? (mol. wt. of X is ...

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... if it was a dilution that was measured. What you've calculated (almost, anyway) is the concentration (in moles per L or molarity) of NADH in the cuvette . If you really mean to calculate "micromoles per minute," like you might if you were calculating a clearance rate of a drug, for ...

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... based on the given information. First, the Beer's law equation is A=c(epsilon)l. I assume that you have a 1cm light path length through your cuvette and that your figure for epsilon (the absorbance of a 1 mg/ml solution) is calibrated for a 1 cm path length and so epsilon has units of (mg/ml)^-1 ...

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... solution for an enzyme assay. To do that you need to make a suitable dilution of the stock solution so that the final expected absorbance in the cuvette will fall in the range of 0.1 to 1 A. Suggest a suitable dilution. Use the wavelength with the extinction of 2375 for the calculation, but you ...

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... obligation to answer if you don't want to--how would your answer change if, given the same extinction coefficient and observed absorbance in the cuvette, the dilution instead was: 1 ml tryptophan stock solution + 19 ml water; or how about 1 ml tryptophan stock solution + 99 ml of water?

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