Cutting site

... the front of the band. And different restiction enzyme have different cutting site, so likely different fragment size: think of divinding a book in fragments everytime a ...

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... information in the articles, stories and commentaries posted on this site range from cutting edge hard news and comment to extreme and unusual perspectives. We choose not to sweep ...

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... information in the articles, stories and commentaries posted on this site range from cutting edge hard news and comment to extreme and unusual perspectives. We choose not to sweep ...

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... it can be inserted in only one direction. I have never heard of CAP sites, but I know that some restriction enzyme are more efficient when there are overhang (the size depends on the enzyme) before the cutting site. I suspect that what you want is to insert 2 different restriction ...

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What if the fragment of interest is bound by one set of restriction sites, but those restriction sites are not present in the plasmid? Using a ... to get the fragment into the plasmid, even if you lose the option of cutting the fragment out at the same positions. If you planned well and inserted ...

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