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Cutting site

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Restrction digest on a plasmid--how many fragments?

... gene of interest that I wish to clone into it and then GOI has a EcoRI site at N-terminal and BamHI at C-terminal...so I would cut my GOI with those ... gel.. I've have searched for this online..but exmaples only talk about cutting the plasmid with one enzyme cheers, b_06er

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by biology_06er
Sat Jun 16, 2012 12:41 am
Forum: General Discussion
Topic: Restrction digest on a plasmid--how many fragments?
Replies: 1
Views: 1861


... at cag tCC ATG gga aca ggc tca caa g [CCATGG is NcoI restriction site] Reverse primer: tat GTCGACTTA TTC GTG CCA TTC GAT TTT CTG AGC CTC GAA ... Avitag to C-terminal of my protein, so I designed in that way with 2 RE cutting site. However, after PCR, double digestion, ligation and transformation, ...

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by akane19861020
Thu Dec 15, 2011 9:39 am
Forum: Molecular Biology
Topic: PCR
Replies: 3
Views: 1379

restriction enzyme question.

... ends (right?)...Now what I don't get is the "finding" of the cutting site within the donor DNA...how come textbooks/online information assume their will be a cutting ...

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by biology_06er
Fri Jun 17, 2011 1:24 pm
Forum: General Discussion
Topic: restriction enzyme question.
Replies: 9
Views: 4613

Re: Sound and how the brain reacts to it

... sensation. However, I find that I relate to the comments on this site more than the others I have reviewed. The depictions of everyone's personal ... along the floor as he/she works. I've found video clips of hair cutting online and I can almost completely drift away while I listen to them. ...

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by sounds
Sun Mar 27, 2011 2:44 am
Forum: Human Biology
Topic: Sound and how the brain reacts to it
Replies: 89
Views: 146149

Molecular Genetics Questions!

Ok anybody that can help me out with all this cutting/ligating stuff is greatly appreciated! I guess I need some help with either links/books, etc. Please explain! Thanks in advance!! 8. What new cleavage site(s) is (are) created when DNA is cut with HindIII, filled in by DNA polymerase ...

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by prenurse
Wed Jan 26, 2011 7:49 pm
Forum: Genetics
Topic: Molecular Genetics Questions!
Replies: 1
Views: 1582
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