Cut

... am having a bit of a difficult time finding actual journal articles on the study. Does anyone have any suggestions? Mandelbrot's book just doesn't cut it.

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If it's in snails, can't you simply use many of them and cut few of them at each time point?

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I think this is a case where winding the DNA onto histones protects it from the nuclease, so the DNA is cut only in regions between the histones. If the same length of DNA is wound around each histone and every DNA segment between histones gets cut, the fragments after inter-histone ...

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... micrococcal nuclease within the nucleus and studying it by SDS-PAGE.The results we had, showed several bands around 6 (nucleosomes that were cut by the nuclease). I do not really know how to interpret the results caz I do not understand what they mean by periodicity (this is supposed to analise ...

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... or dephosphorylate any of the two? As far as I understand, it is not necessary to dephosphorylate my double-digested open vector as long as it was cut with two different enzymes. Thus, the 5'-end of my vector has a phosphate. But still, should I phosphorylate my insert, since its 5'-end does not ...

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