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Curves

Curve

a nonangular deviation from a straight course in a line or surface.


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amplification in no RT control

... of my no RT controls (4 treatments and an untreated), although this is occurring ~20 cycles later than RT+ samples. I am getting single peak melt curves and my NTC is not amplifying. This suggests to me that I am not getting dimers and my reagents are 'good' I have tried cDNA at 10ng and 5ng and ...

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by biomart
Fri Mar 20, 2015 1:58 am
 
Forum: General Discussion
Topic: amplification in no RT control
Replies: 0
Views: 34

"dose"-response curves

I have always wondered why dose-response curves are constructed against the concentration of a drug rather than the amount of a drug. Typically, an effect increases or decreases with increasing concentration in g/l or mol/l. Why not graphing ...

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by Mathias
Wed Feb 25, 2015 3:23 pm
 
Forum: Cell Biology
Topic: "dose"-response curves
Replies: 0
Views: 254

Minimum Data Points for elisa standard curve

... that the company gave me, it is noted that the calibration curve should be a linear plot. But my results can be better explained by non linear curves. Am I allowed to disregard test instruction in this case? Any help would be appreciated.

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by rgm
Sun Feb 01, 2015 7:19 am
 
Forum: Molecular Biology
Topic: Minimum Data Points for elisa standard curve
Replies: 0
Views: 299

Re: Brain size=IQ level theory (Blacks vs Whites & Asians)

... it's pretty obvious that I'm smarter than you. Hate to break it to you. :mrgreen: I also alluded to the frequency distribution curve. Distribution curves don't reflect genetics. IQ can be learned. IQ scores are not frozen in time for all eternity. IQ rises and IQ gaps close. So, again, what is ...

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by Seth90210
Sun Feb 16, 2014 9:35 pm
 
Forum: Human Biology
Topic: Brain size=IQ level theory (Blacks vs Whites & Asians)
Replies: 75
Views: 332504

TLDA screening analysis

... a Workflow in R for analyzing exported .txt files, but we haven't software to analyze all the plates at once in order to analyze the amplification curves. There is software to scan files .sds (Applied Biosystems qPCR device), but not files .eds (new file format exported by Viia7). 2 questions: ...

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by laurentPre
Fri Jan 17, 2014 12:59 pm
 
Forum: Molecular Biology
Topic: TLDA screening analysis
Replies: 4
Views: 1909
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