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Cumulus

Cumulus

a collection or heap of cells.

Origin: L. A heap


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Questions about single pig oocyte RT-PCR.

... 2ul RNase Inhibitor 5ul dNTP 10ul oligodT 10ul DTT 10ul 5*RT buffer 40ul Add H2O to 100ul, Take 5ul to eppendorf tube. hyaluronidase to remove cumulus cells. The amplification program for the reverse transcription step was as follows: 70°C for 10 min, ice 2min ,MMLV ,42°C for 50min, 95°C for ...

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by dragonwang
Mon Jun 23, 2008 3:48 am
 
Forum: Molecular Biology
Topic: Questions about single pig oocyte RT-PCR.
Replies: 1
Views: 1349


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