Cumulus

... 2ul RNase Inhibitor 5ul dNTP 10ul oligodT 10ul DTT 10ul 5*RT buffer 40ul Add H2O to 100ul， Take 5ul to eppendorf tube. hyaluronidase to remove cumulus cells. The amplification program for the reverse transcription step was as follows: 70°C for 10 min, ice 2min ,MMLV ,42°C for 50min, 95°C for ...

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