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(Science: cell culture) The process of incubating viruses or bacteria from tissue or other body fluids, for the purpose of identification.

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western blotting of DNA-binding protein of mammalian cell

i am trying to immunoblot one of the DNA-binding protein.... first i have cultured the cells in 25 cm2 flask and collected the cell lysate using RIPA lysis (1 ml) buffer...kept in ice for 5 min, followed by passing the lysate through 24 G needle about 5 times...then ...

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by drelamuruganvet
Mon Jan 19, 2015 9:39 am
Forum: Molecular Biology
Topic: western blotting of DNA-binding protein of mammalian cell
Replies: 0
Views: 430

Please help me decide which microscope to buy

... and IF you only had enough money for one of these (both at around the same price), would you go for the inverted or compound micoscope for viewing CULTURED CELLS. One of my worries is that 430X might not be enough, but I haven't done any research on human cells. For anyone doing reseach on cell ...

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by NathanielZhu
Sat Nov 02, 2013 8:54 pm
Forum: General Discussion
Topic: Please help me decide which microscope to buy
Replies: 2
Views: 2800

Re: seahorse

It is a machine that measures the respiration of your tissue cultured cells, the website is http://www.seahorsebio.com/ This is really cool technology, we have one machine on campus

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by Nstull
Thu Feb 28, 2013 7:40 pm
Forum: Molecular Biology
Topic: seahorse
Replies: 3
Views: 2118

Human Pathology 'Multiple-Choice Questions'

... 9. Granulosa cells are aspirated from a dominant follicle of a 25 year-old XX subject on day 11 of the menstrual cycle. Granulosa cells are then cultured in vitro, in the presence of LH and FSH. At the 3rd day of culture, estrogen secretion by these cells would: a. skyrocket to surge levels comparable ...

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by ScrimmSphere
Mon Feb 04, 2013 11:03 am
Forum: Human Biology
Topic: Human Pathology 'Multiple-Choice Questions'
Replies: 1
Views: 6986

Bacterial Calculation

Hi I am trying to calculate colony forming units calculation. I cultured bacteria overnight (15ml) and did O.D reading at 0.4 and did serial dilution (10*1 to 10*9) and I got 55 colonies at 10*4 dilution. So I got 5.5 × 10*6 cfu/ml (original, if I am right). ...

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by chandch
Thu Nov 15, 2012 6:03 pm
Forum: Microbiology
Topic: Bacterial Calculation
Replies: 3
Views: 3963
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