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Culture medium

Culture medium

a substance, either solid or liquid, used for the cultivation, isolation, identification, or storage of microorganisms.

Synonym: growth medium, medium, nutrient medium.


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Re: sterilization in microwave oven

... of 'inspissation' came into use after the turn of the 20th century. A culture medium would be heated to boiling and then held at room temperature or even incubated for a ...

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by fdgsr
Tue Mar 18, 2014 3:13 pm
 
Forum: Microbiology
Topic: sterilization in microwave oven
Replies: 29
Views: 102845

URGENT HELP IDENTIFYING UNKNOWN BACTERIA!!!

... cells. SPORE REACTION (-) ACID FAST STAINING (-) NUTRIENT BROTH CULTURE- Quite turbid, slightly cloudy, no skin NUTRIENT AGAR STREAK- Shape ... NUTRIENT AGAR STAB: Aerobic GELATIN STAB: No Proteolysis THIOGLYCOLLATE MEDIUM- Anaerobic SEMI SOLID (Sloppy) Agar – Anaerobic BLOOD AGAR PLATE: ...

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by microbiologyhelp
Wed Nov 20, 2013 6:09 pm
 
Forum: Microbiology
Topic: URGENT HELP IDENTIFYING UNKNOWN BACTERIA!!!
Replies: 7
Views: 6188

Cell culture - no CO2

... posts). You may have success if you closely monitor the pH of your culture medium and adjust it when required during your culture. Normal cultures typically require maintenance ...

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by biohazard
Mon Sep 23, 2013 9:01 am
 
Forum: Cell Biology
Topic: Cell culture - no CO2
Replies: 10
Views: 21230

Problems making chemically competent cells

... cold room, (and maybe an eppendorf rack in the -80) 1. Make a 5ml pre-culture overnight from a single colony, in SOC medium 2. The next day, inoculate 100ml pre-warmed SOC medium with 5ml of the overnight pre-culture. ...

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by lilruthc
Fri Feb 08, 2013 1:09 pm
 
Forum: Molecular Biology
Topic: Problems making chemically competent cells
Replies: 1
Views: 2657

peripheral mononuclear cells handling

... of the scraper). The monocytes require an hour or two in +37C in culture medium to attach to the plate surface, and thus after 24h they should be firmly stuck and you ...

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by biohazard
Mon Nov 05, 2012 1:11 pm
 
Forum: Cell Biology
Topic: peripheral mononuclear cells handling
Replies: 3
Views: 2089
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