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Culture media

Culture media

Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as agar or gelatin.


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Re: sterilization in microwave oven

... of 'inspissation' came into use after the turn of the 20th century. A culture medium would be heated to boiling and then held at room temperature ... obtain almost absolute sterility. Most early bacteriology was done with media 'sterilized' in that manner. Microwave could be used in the same way ...

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by fdgsr
Tue Mar 18, 2014 3:13 pm
 
Forum: Microbiology
Topic: sterilization in microwave oven
Replies: 29
Views: 101648

Re: Please help me decide which microscope to buy

... (plastic-thickness) to the cells without dumping the objective into the culture media. This will probably limit the maximum magnification you can use, because the short focal ...

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by jonmoulton
Wed Nov 06, 2013 8:00 pm
 
Forum: General Discussion
Topic: Please help me decide which microscope to buy
Replies: 2
Views: 1580

Where to get Fibroblasts and Immune stem cells?

I'm wanting to get some simple adult stem cells to culture and play around with but I don't want to pay the heafty price for professional ... stem cells (which I theoretically know how to do and I know where to get the media). So....I'm wondering, is it possible to get fibroblasts and blood stem ...

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by NathanielZhu
Wed Nov 06, 2013 5:07 am
 
Forum: General Discussion
Topic: Where to get Fibroblasts and Immune stem cells?
Replies: 0
Views: 594

Culturing or viewing cells

2. Different cancer cells will definitely require different media. But the general procedure assuming you need to get cells from a particular ... extract the cells, digest ECM..., try culturing them in a flask / Tissue culture plate (depending on the type of cells it can be determined if anchoring ...

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by jwalin
Fri Aug 30, 2013 2:22 pm
 
Forum: Cell Biology
Topic: Culturing or viewing cells
Replies: 3
Views: 3302

Cells hyper-sensitive to treatment after 48 hour culture

... cells show no signs of dying (annexin V + propidium iodide staining) and are morphologically healthy. I've bought and made up new batches of media, ensured my supplements were the same lot number. I've also checked for mycoplasma infection and that has come up negative and still I'm having ...

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by danint
Thu Sep 20, 2012 2:20 pm
 
Forum: General Discussion
Topic: Cells hyper-sensitive to treatment after 48 hour culture
Replies: 0
Views: 910
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