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Csf

Csf

1. Colony-stimulating factor.

2. Cerebrospinal fluid.


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Learning tissue culture, specifically moDC generation

... surface (they attach readily to most "hard" plastic surfaces), serum-free culture medium (such as AIM-V), growth factors (poly-I:C, GM-CSF) and an incubator. Toss freshly isolated PBMCs on a properly sized plate (e.g. 24-well plate) in AIM-V, incubate for, say, 2 hours at +37C and wash ...

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by biohazard
Fri Feb 03, 2012 2:09 pm
 
Forum: Cell Biology
Topic: Learning tissue culture, specifically moDC generation
Replies: 2
Views: 2165

total RNA extraction from human cerebrospinal fluid (CSF)

Hi, I am looking for a kit (protocol) for total RNA extraction from cerebrospinal fluid (CSF). I will deeply appreciate any advice on this issue. Also, what will be the RNA yield to look for and if there is a biorepository from where I can buy some CSF samples?. Thank ...

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by lcastillos
Fri Oct 17, 2008 6:49 pm
 
Forum: Molecular Biology
Topic: total RNA extraction from human cerebrospinal fluid (CSF)
Replies: 0
Views: 1633

lysozyme doubt...

It will kill bacterial cells present in CSF. No effect on animal cells

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by pankaaj
Fri Mar 14, 2008 10:03 am
 
Forum: Molecular Biology
Topic: lysozyme doubt...
Replies: 2
Views: 1706

lysozyme doubt...

agar LYSOZYME csf me hota to kya hota?

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by sonawanes61
Fri Mar 14, 2008 9:59 am
 
Forum: Molecular Biology
Topic: lysozyme doubt...
Replies: 2
Views: 1706

Resuspending cytokines - Carrier proteins

Hi all, I have just purchased some cytokines (m-CSF and RANKL) from a commercial supplier. They are supplied lyophilized and without carrier protien. To store @ -20deg, the product sheet says to resuspend in water with a carrier protein. Does anyone ...

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by Beaker73
Mon Jul 30, 2007 4:35 am
 
Forum: Cell Biology
Topic: Resuspending cytokines - Carrier proteins
Replies: 0
Views: 1038
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