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Dictionary » C » Csf Csf![]()
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Results from our forumLearning tissue culture, specifically moDC generation... surface (they attach readily to most "hard" plastic surfaces), serum-free culture medium (such as AIM-V), growth factors (poly-I:C, GM-CSF) and an incubator. Toss freshly isolated PBMCs on a properly sized plate (e.g. 24-well plate) in AIM-V, incubate for, say, 2 hours at +37C and wash ...
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total RNA extraction from human cerebrospinal fluid (CSF)Hi, I am looking for a kit (protocol) for total RNA extraction from cerebrospinal fluid (CSF). I will deeply appreciate any advice on this issue. Also, what will be the RNA yield to look for and if there is a biorepository from where I can buy some CSF samples?. Thank ...
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Resuspending cytokines - Carrier proteinsHi all, I have just purchased some cytokines (m-CSF and RANKL) from a commercial supplier. They are supplied lyophilized and without carrier protien. To store @ -20deg, the product sheet says to resuspend in water with a carrier protein. Does anyone ...
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