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Cryoprotectant

Cryoprotectant

(Science: cell biology) substance that is used to protect from the effects of freezing, largely by preventing large ice crystals from forming. The two commonly used for freezing cells are dmso or glycerol.


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Freezing Het-1A cells

I recently obtain via ATCC Het-1A cells. Their product info sheet says that I must use the following cryoprotectant medium: L-15 plus 10% FBS, 1% PVP, and 7.5% DMSO. I did order the specific complete growth medium BEGM kit but I want to know if I can just DMSO to this growth ...

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by lrarias
Mon Oct 26, 2009 5:40 pm
 
Forum: Cell Biology
Topic: Freezing Het-1A cells
Replies: 3
Views: 1878

Cellular Banking or white collar Crime ??

... cells for 21 years . Can anyone please tell if there is substantial amount of documentation that proves that the cells remains viable even if the cryoprotectant like hespan is added. Liquid nitrogen will definitely cease the biological activity of the cell but will it NOT hamper the viability ...

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by ManishaSapre
Mon Sep 28, 2009 10:50 am
 
Forum: General Discussion
Topic: Cellular Banking or white collar Crime ??
Replies: 1
Views: 1151

plant and cold stress

... of genetic program to be able to survive to cold temperature. exemples of this regulation are to permit synthesis of proteins and sugar who are cryoprotectant (chaperonin, ....), to change the membrane fluidity membranes are sensitive to low temperature, because low temperatures reduce membrane ...

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by fabienne
Wed Aug 10, 2005 10:16 am
 
Forum: Botany Discussion
Topic: resit question-please help....
Replies: 4
Views: 3254


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