Crying

Hi there, I have to do a cell wall extraction on bacteria soon and my protein of interest is cell wall anchored. My supervisor told me to do TCA precipitation (haven't done before) but I assume it's too isolate the proteins within the cell wall?? He told me I would have three components by the end o...

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anybody no idea? :cry:

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... my midi prep(Keratinase sequence)and Maxi Prep(Vector) is showing the same band... I run the agarose at 70v 85mamp for 2hrs... I really feel like crying,,, Kindly tell me what to do, should I make the restriction again on monday, and also check with the PCR about my sequence... please give me ...

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1. While under the influence of cocaine, some users report prolonged feelings of pleasure because dopamine a) reduces the refractory period of neurons b) remains in the synapse longer than normal c) increases the speed of nerve impulse transmission d) diffuses rapidly throughout the pleasure centres...

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Hi my name is Alexandria and i have two different colored eyes. One is green and one is blue. Sometimes i wish i just had blue eyes because they're pretty and amazing. Plus, people say thats prettier. I'm a model. Sometimes its really hard work. Anyway, i have two different color eyes!! :cry: yay!!

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