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Cross reaction

Cross reaction

(Science: haematology, immunology) serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.

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Ligase reaction problem

... below. Here, I want to ask whether it is enough base lenght for ligase reaction via thermostable ligase in 63 C (Protocol: 5 cycle at 95 C-30 s and 63 C-4 min) I think I take cross reaction because of this dimer but I am not sure. Is it possible to discard this ...

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by Molbio34
Thu Feb 26, 2015 8:27 am
Forum: Molecular Biology
Topic: Ligase reaction problem
Replies: 0
Views: 323

Improving qPCR assay detection limits

... perfectly in the range 10^8 – 10^4 copies of either antibody chain per reaction (acceptable efficiency, Pearson correlation, amplification is every ... 10-10^3 standards are added to the standard curve, however, they all cross the threshold line at about 24 cycles along with the 10^4 standard, ...

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by Hriss
Thu May 12, 2011 10:06 am
Forum: Molecular Biology
Topic: Improving qPCR assay detection limits
Replies: 0
Views: 1141

Denaturation and refolding of an enzyme

... them. lysozyme retains the antibacterial activity and stabileses cross linkages. The presence of disulphide bonds make the protein less suspectible ... the question? Q2.What is the function of cysteine in the renaturation reaction? I think the answer : The side chain on cysteine is thiol participates ...

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by enzsel
Thu Dec 02, 2010 1:54 pm
Forum: Cell Biology
Topic: Denaturation and refolding of an enzyme
Replies: 3
Views: 4104

Gluten-free and Vaccines

... but you will notice simply that they prove that both can elicit similar reaction. NOT that gliadin will necessarily cause an immunological cross reaction between the 2 peptides. And there is a difference between the inability to digest ...

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by canalon
Tue Jun 22, 2010 8:37 pm
Forum: General Discussion
Topic: Gluten-free and Vaccines
Replies: 7
Views: 20099

Biology IQ test: How smart are you = look

... because their conversion into graphite has a G>0 4) Enzymes speed reaction rates by facilitating the development of the transition state. 5) ... kinase cascades must A. never occur in cells B. by definition engage in cross talk C. be very unlikely since there are only two kinase pathways in ...

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by DemiMike
Sat Nov 28, 2009 6:21 am
Forum: General Discussion
Topic: Biology IQ test: How smart are you = look
Replies: 3
Views: 13948
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