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Coverslip

Coverslip

(Science: microscopy) thin glass, plastic, sapphire, SiO, NaCl, etc. Shaped into circles, squares, or rectangles for covering the specimen. Glass coverslips, grade no. 1 1/2, contain a high proportion of covers from 0.17 to 0.18 mm in thickness, close to the lens designer's specifications for employment of achromatic and apochromatic objectives of high numerical aperture. The thickness and refractive index of the glass coverslip are important because certain of their values have been assumed by the designer and manufacturer. These values have been specified by ASTM: thickness, commercial number 1 1/2: 0.16 to 0.19 mm, refractive index measured at the sodium D lines (589.3 nm), 1.523(0.005).


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Tips for staining (light microscopy)

my tip about reducing bubbles is. Use a needle. Hold the coverslip with your left hand, hold the needle with your right hand. Place the edge of a coverslip next to sample in sharp angle and support the oposite edge with a needle. Go down (slowly) with ...

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by triton
Wed Aug 22, 2012 8:33 am
 
Forum: Microbiology
Topic: Tips for staining (light microscopy)
Replies: 3
Views: 4071

Re: Tips for staining (light microscopy)

... reply to all these questions, but I'm probably not as experienced as some here. 1) The way I minimize bubbles on my wet mounts is by holding the coverslip at a 45° angle and placing the edge of the coverslip on the drop of liquid. This will draw the liquid fully across the edge of the slip. You ...

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by PsycoCarrot
Fri Jul 06, 2012 11:03 pm
 
Forum: Microbiology
Topic: Tips for staining (light microscopy)
Replies: 3
Views: 4071

HELP! with ethidium bromide experiment

... of the nucleus. Any other ideas for the other 2 questions? Please and thanks!!! 3. Researchers grew mammalian tissue culture cells on three coverslips. Each of the coverslips was treated, under identical conditions, with detergent to remove all membranes and with high salt buffers to remove ...

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by BritKels
Thu Mar 03, 2011 12:23 am
 
Forum: Molecular Biology
Topic: HELP! with ethidium bromide experiment
Replies: 15
Views: 8646

Polynculeation

I have a culture of Mouse fibroblast cells. When I seed them on to a glass coverslip and observe them in an inverted microscope I see lots of cells with 2 or 3 nuclei and sometimes the nuclei appear to be enlarged. What is going on? Please help a lowly biophysicist. ...

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by softmatters
Thu Jun 04, 2009 2:28 pm
 
Forum: Cell Biology
Topic: Polynculeation
Replies: 1
Views: 1423

diffusion and osmosis. help me please?

... [not sure] the water moved out of the cell because it moves from area of high concentration to areas of low concentration 8. now, remove the coverslip and thoroughly rinse the elodea leaf under tap water. prepare a new slide of the same leaf using distilled water. look for changes in the ...

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by lanzshade
Thu Oct 02, 2008 12:50 am
 
Forum: Cell Biology
Topic: diffusion and osmosis. help me please?
Replies: 1
Views: 14316
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