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Coverslip

Coverslip

(Science: microscopy) thin glass, plastic, sapphire, SiO, NaCl, etc. Shaped into circles, squares, or rectangles for covering the specimen. Glass coverslips, grade no. 1 1/2, contain a high proportion of covers from 0.17 to 0.18 mm in thickness, close to the lens designer's specifications for employment of achromatic and apochromatic objectives of high numerical aperture. The thickness and refractive index of the glass coverslip are important because certain of their values have been assumed by the designer and manufacturer. These values have been specified by ASTM: thickness, commercial number 1 1/2: 0.16 to 0.19 mm, refractive index measured at the sodium D lines (589.3 nm), 1.523(0.005).


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Polynculeation

I have a culture of Mouse fibroblast cells. When I seed them on to a glass coverslip and observe them in an inverted microscope I see lots of cells with 2 or 3 nuclei and sometimes the nuclei appear to be enlarged. What is going on? Please help a lowly biophysicist. ...

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by softmatters
Thu Jun 04, 2009 2:28 pm
 
Forum: Cell Biology
Topic: Polynculeation
Replies: 1
Views: 118

diffusion and osmosis. help me please?

... [not sure] the water moved out of the cell because it moves from area of high concentration to areas of low concentration 8. now, remove the coverslip and thoroughly rinse the elodea leaf under tap water. prepare a new slide of the same leaf using distilled water. look for changes in the ...

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by lanzshade
Thu Oct 02, 2008 12:50 am
 
Forum: Cell Biology
Topic: diffusion and osmosis. help me please?
Replies: 1
Views: 2757

hanging drop..

... and if they bear any locomotory organelle, say flagella for instance, will try to move to the edges to escape the anaerobic conditions inside that coverslip. So, motile bacteria are often found on the edges to recieve oxygen.

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by monny
Fri Nov 09, 2007 12:45 pm
 
Forum: Microbiology
Topic: hanging drop..
Replies: 2
Views: 1054

Tetrad Analysis

... say to placea few perithecia (those are the rod shaped structures containing the spores right?) on microscope slide etc etc and cover with coverslip...then it goes on say examine as many asci and record the number arising by 1st division segregation as opposed to 2nd division segregation ...

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by biology_06er
Tue Jul 31, 2007 5:50 am
 
Forum: Genetics
Topic: Tetrad Analysis
Replies: 0
Views: 659

effect of temperature on the survival of yeast cells

... desired temperature, use a pipette and put one drop of the mixture into a haemocytometer slide (or a normal one, if you so wish). Once you put a coverslip on, put 2 drops of methylene blue at one end of the coverslip (again, using a pipette). Then, once there's 2 drops (they should be just on ...

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by Ulbrid
Sat Apr 28, 2007 3:19 pm
 
Forum: Cell Biology
Topic: effect of temperature on the survival of yeast cells
Replies: 241
Views: 64136
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