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Coverage

Coverage

a measure of the extent to which the services rendered cover the potential need for these services in a community; applied specifically to such services as immunization in developing countries.


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How can a protein without a G-site bind to GSH?

3% coverage is not very high, what was your score? That was the only detected protein?

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by JackBean
Mon Nov 12, 2012 3:01 pm
 
Forum: Molecular Biology
Topic: How can a protein without a G-site bind to GSH?
Replies: 11
Views: 3407

How can a protein without a G-site bind to GSH?

... I used GSH-Sepharose 4B beads on a cell lysate and got a single protein band on SDS-PAGE. I sent it for MALDI-TOF, the sequence came back with 3% coverage that has homolog with acetyl carrier protein. question: 1. how can my protein bind to GSH without having a G-site? 2. is it a case of bad prep? ...

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by hurzz
Mon Nov 12, 2012 12:34 pm
 
Forum: Molecular Biology
Topic: How can a protein without a G-site bind to GSH?
Replies: 11
Views: 3407

Sequencing question/primer design

... I can do). However, he also wants me to determine sequencing primers (ones which will be in the middle of the sequence to provide complete gene coverage). There are 2 questions he is asking that confuses me. 1) Design a forward primer for XXX gene sequence that is in frame (in frame as in the ...

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by coppcw08
Sun Nov 04, 2012 8:19 pm
 
Forum: Molecular Biology
Topic: Sequencing question/primer design
Replies: 4
Views: 3188

Re: Telepathy - it works or maybe not?

I don’t believe to such thing before until I saw one on the national television. Though the coverage is not focused in telepathy, it is about the mentalism; the guy was able to know what’s on the guest mind. He is asking questions and let the person think of the answer ...

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by rihannapark
Mon Nov 28, 2011 6:49 am
 
Forum: General Discussion
Topic: Telepathy - it works or maybe not?
Replies: 9
Views: 4544

E value confidence

If there's only 2% coverage of 1 kb, that means only 20 similar nts!!! In such a case you cannot take that as significant (that short part may be, but not the whole region)

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by JackBean
Mon Dec 20, 2010 2:10 pm
 
Forum: Bioinformatics
Topic: E value confidence
Replies: 4
Views: 2446
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