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Coupling

Coupling

(Science: biochemistry) The linking of two independent processes by a common intermediate, for example the coupling of electron transport to oxidative phosphorylation or the atp adp conversion to transport processes.


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Two-color in situ help

... is Fluorescein labeled) were added to the sample and hybridized at 65C overnight. I use anti-Digoxigenin-POD for the first antibody reaction, coupling it with cy3 (red) for the TSA reaction. Then I use PH2.2 Glycine + tween20 to disable the first AB and wash many times. Then I add the second ...

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by vastgenome
Thu Feb 14, 2013 5:00 am
 
Forum: Molecular Biology
Topic: Two-color in situ help
Replies: 3
Views: 1620

ETC - purpose of having different dehydrogenases?

... have different NADH dehydrogenase complexes (more specifically, NDH-1 and NDH-2). One major difference between the two is the fact that NDH-1 is a coupling site (so pumps out protons while transferring electrons) while NDH-2 is not; will not pump out any protons. As a result of this, the NET ATP ...

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by keetner
Sat Dec 15, 2012 2:28 am
 
Forum: Molecular Biology
Topic: ETC - purpose of having different dehydrogenases?
Replies: 1
Views: 1683

Re: connexins & pannexins

... gap junction proteins, connexins, innexins and pannexins, emphasizing their importance as intercellular channels that provide ionic and metabolic coupling and as non-junctional channels that can function as a paracrine signaling pathway. We discuss that two distinct groups of proteins form gap ...

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by brad000123
Sat Oct 20, 2012 11:54 am
 
Forum: Molecular Biology
Topic: connexins & pannexins
Replies: 6
Views: 5277

Explain why Genetic Code is called "Rosetta Stone" of life?

You seem to understand the principles of the genetic code. But, sorry, you failed coupling it to the Rosetta stone. Hope you passed the test. This stone appeared to be the key to understand several languages. Just as the genetic code is the key to understand the ...

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by citroenboom
Tue Jul 17, 2012 7:26 am
 
Forum: Genetics
Topic: Explain why Genetic Code is called "Rosetta Stone" of life?
Replies: 11
Views: 6830

Re: Ventilation - Perfusion Coupling

Thanks anyway. The exam is in an hour and I still don't "get it," but I'll just have to hope for the best!

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by lalajane
Mon May 23, 2011 11:19 am
 
Forum: Cell Biology
Topic: Ventilation - Perfusion Coupling
Replies: 2
Views: 4753
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