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Contiguous map

Contiguous map

(Science: molecular biology) a map depicting the relative order of a linked library of small overlapping clones representing a complete chromosomal segment.


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Sequencing

You look at the fragments and then you pick areas where they overlap, given enough copies you should be able to build a contiguous consensus based on overlaps. Theoretically you could have a bunch of frags as short as 4bps long and still be able to get a decent length of sequence.

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by mith
Fri Apr 17, 2009 5:03 pm
 
Forum: Bioinformatics
Topic: Sequencing
Replies: 5
Views: 647

E-PCR confusion

... and mapped tagged sequences in a particular genome. The genomic sequence would refer to the DNA sequence, which may or may not (probably won't) be contiguous relative to its mRNA. The mRNA corresponds to the spliced message and will be a contiguous sequence. The EST is the original tagged sequence.

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by blcr11
Sun Aug 05, 2007 11:52 am
 
Forum: Bioinformatics
Topic: E-PCR confusion
Replies: 1
Views: 1394

The Fiber Disease

... from glycine, essentially under microaerophilic conditions. The genetic basis of HCN synthesis in P. fluorescens CHA0 was investigated. The contiguous structural genes hcnABC encoding HCN synthase were expressed from the T7 promoter in Escherichia coli, resulting in HCN production in this ...

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by Skytroll
Tue Oct 24, 2006 6:21 pm
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 749218

The Fiber Disease

... the physical injection of the transgene into the target cells of the host insect. But, the chromosomes in the nuclei of cells are each single, contiguous, very long strands of DNA. For a transgene to become part of a chromosome, the chromosome must be completely broken into two pieces and the ...

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by Skytroll
Sat Aug 19, 2006 2:16 am
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 749218

The Fiber Disease

... clustering in some areas, and because of a characteristic preceding skin lesion in some patients. The authors studied 51 residents of three contiguous Connecticut communities -- 39 children and 12 adults -- who developed an illness characterized by recurrent attacks of asymmetric swelling ...

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by John Kern
Tue Apr 18, 2006 1:03 am
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 749218
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