Contamination

Have you checked for contamination in your reagents by using the kits on a previously tested primer that you know gives the right product size? My lab recently had an issue with contamination in our SuperScript mix that was giving us ...

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... What happens is that you kill most of the microorganism, and since this is repeated daily, and considering that the eyes can deal with limited contamination it is probably safe enough, but the contact lenses are probably not sterile. - Microwave can and do superheat water above 100ºC, but this ...

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... did you use more than 2 primers? which polymerase did you use (proofreading or not)? does a primer anneal to the mutated site? I don't think that contamination is the explanation to that. I'd expect more bands not too less if you had a contamination. In my opinion the only thinkable explanation ...

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... Can anyone help me as to what kind of things can go wrong with PCR and agarose gel electrophoresis experiments. The only thing I can think of is contamination of DNA samples but there must be other reasons. I would be grateful if someone can help. Thanks

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Hi everyone: I want to isolate the buffycoat layer (MNCs) from a peripheral blood sample? How can I make to get a pure layer without red cell contamination. I have tried A Ficol solution but there was a contamination with the ficol particles so any one can help ???

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