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Contaminants


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Re: sterilization in microwave oven

... a job if married because their husbands were their owners and could veto any order by a super seer.) Of course anthrax spores and spores of common contaminants were not killed. But, the rare spore of a pathogen on an instrument after it was thoroughly washed and dried along with the improbability ...

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by fdgsr
Tue Mar 18, 2014 3:13 pm
 
Forum: Microbiology
Topic: sterilization in microwave oven
Replies: 29
Views: 93764

How to maintain sterility while preparing petri dishes

... I think I've found where I've gone wrong—the agar itself. I only boiled it for a few minutes each time I've tried so there were very likely some contaminants still in there. After I used the bottle the first time, there were some strange things growing on the surface. It had a greenish, mucousy ...

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by animus
Thu Jun 27, 2013 12:18 pm
 
Forum: Microbiology
Topic: How to maintain sterility while preparing petri dishes
Replies: 8
Views: 2260

How to maintain sterility while preparing petri dishes

... minutes, you are likely to get no contaminating bacteria from the air (our 100+ students do so dozens of times in our labs and we see airborne contaminants very, very rarely - the contamination is almost always from poorly sterilized loops or from their own butter-fingers!). That being said, ...

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by biohazard
Mon Jun 24, 2013 8:56 am
 
Forum: Microbiology
Topic: How to maintain sterility while preparing petri dishes
Replies: 8
Views: 2260

How to maintain sterility while preparing petri dishes

... In addition to that, pouring the agar should be done in a laminar flow hood, which essentially eliminates the issue of airborne bacteria or other contaminants. For some of our courses we prepare like 1000 agar plates this way and in most cases none of them have signs of contamination. When the ...

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by biohazard
Mon Jun 24, 2013 7:12 am
 
Forum: Microbiology
Topic: How to maintain sterility while preparing petri dishes
Replies: 8
Views: 2260

Re: Question re: storing bacteria sample

... glycerol from your local pharmacy (don't worry about the lack of sterility - if your sample contains nasties, they will still be there even if contaminants are there also), put your scrapings into an airtight container, add some glycerol, and freeze it until you can have it checked.

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by wbla3335
Sun Aug 26, 2012 4:01 am
 
Forum: General Discussion
Topic: Question re: storing bacteria sample
Replies: 2
Views: 2271
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