Contaminants

Ok basicly its just that some species of fish have been around and taken in certain "bacteria and contaminants" that have created a intersexual devolopement. SO this brings the interesting fact that one fish can hold both the female and male reproductive parts. heres ...

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... absence of something etc). Your best bet would be to hope that you get exctly what you need and make sure you work sterile so that you don't get contaminants.

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... you thought it was after adding imidazole, I presume. Imidazole, especially 250 mM, can change the pH of your buffer. Imidazole can also carry in contaminants like zinc. If you have a metalloenzyme inhibited by zinc, that could contribute to loss of activity in the presence of imidazole which ...

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... If you do not use antibiotics, you notice the contamination within 24-48 h and can discard the culture. But if the antibiotics inhibit the other contaminants, you may falsely believe that your cells are ok. So that's what Sigma's experts tell us. Personally I feel much safer with antibiotics, ...

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Some people advise to avoid antibiotics, since they render cultures susceptible to so called cryptic contaminants (e.g. mycoplasmas), that have some degree of resistance to these antibiotics and thus are able to slowly grow in tissue/cell culture without causing any visible ...

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