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Contaminant

Contaminant

An impurity; any material of an extraneous nature associated with a chemical, a pharmaceutical preparation, a physiologic principle, or an infectious agent. A substance that contaminates.A foreign species of a given environment where the species is not in its natural habitat, and therefore foreign to the new environment also.


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Re: Microbiology help in identifying Gram + rod for my unknown

... The positive catalase probably points toward Bacillus spp. but taking it to species will be tough. Are you sure you aren't working with a contaminant? The tests you've run seem more relevant to an enteric id excercise. no this is my unknown, it may have been contaminatied, but still I ...

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by ccapelouto838
Wed May 09, 2012 12:09 am
 
Forum: Microbiology
Topic: Microbiology help in identifying Gram + rod for my unknown
Replies: 12
Views: 8564

Re: Microbiology help in identifying Gram + rod for my unknown

... The positive catalase probably points toward Bacillus spp. but taking it to species will be tough. Are you sure you aren't working with a contaminant? The tests you've run seem more relevant to an enteric id excercise.

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by JorgeLobo
Sun May 06, 2012 9:34 am
 
Forum: Microbiology
Topic: Microbiology help in identifying Gram + rod for my unknown
Replies: 12
Views: 8564

MALDI; fusion protein cleaving from a peptide

... visualise it on gel, but I geally worry about MALDI result because it shows nothing at 3,2 kDa.. Honestly it barely shows something... I guess any contaminant may interrupt. Probes contain 1 M urea, 20-50 mM Tris, 1- 2 mM CaCl2, 0,1% Tween20, 100 mM NaCl and some imidasole.. Can any of this reagents ...

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by Valya
Wed Mar 28, 2012 8:42 pm
 
Forum: Molecular Biology
Topic: MALDI; fusion protein cleaving from a peptide
Replies: 6
Views: 2209

SDS PAGE staining box

... lid. Nice polypropylene. Cheap and good size for mini gels. I have a glass container that has been scrubbed acid washed and kept free of as much contaminant as I can for when I need to prepare my gels for silver staining and protein sequencing.

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by canalon
Wed Nov 10, 2010 12:02 am
 
Forum: Molecular Biology
Topic: SDS PAGE staining box
Replies: 3
Views: 2655

Inoculating bacteria

that if you pick one typical colony you are likely to pick a good one, but if you scrape a plate where some contaminant might be lurking, you might miss it in the crowd.

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by canalon
Thu Oct 14, 2010 1:01 am
 
Forum: Microbiology
Topic: Inoculating bacteria
Replies: 5
Views: 4214
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