Constructs

Hello, I have two problems: 1) We ordered pOTB7 vector with gene A from a company and constructed deletion constructs of the gene A from above vector. We designed primers with restriction sites for 4 deletion constructs, PCR amplified and gel purified the fragments using Qiagen ...

See entire post

Hello, I have two problems: 1) We ordered pOTB7 vector with gene A from a company and constructed deletion constructs of the gene A from above vector. We designed primers with restriction sites for 4 deletion constructs, PCR amplified and gel purified the fragments using Qiagen ...

See entire post

... that we can recognize by morphology or genetics (or behaviour). What we choose to call a species is indeed a human construct, but these constructs are based on observation and are just our attempt at categorizing the biological world. You are right in stating that there is no agreed ...

See entire post

... has joined the "ti" to make a "uti". I think you see where I'm going with this, so I won't continue. By keeping stable constructs together and adding to them over time, spelling out "The Theory of Evolution" will happen a little more quickly than you propose ...

See entire post

I've had a problem recently with the E. coli cells I've been using. I transformed 7 constructs into FusionBlue competent cells (from Clontech), they grew many colonies (significantly more than my control). I performed a colony PCR 5 days later and re-streaked the ...

See entire post