Construct

... lists for my unknown bacterial. There are 5 unknown bacteria that I need to identify. I would like to identify the bacteria using blastn and construct phylogenetic tree using neighbor joining. Can anyone help me how to do it, since I not sure and familiar with it. Please help me as fast as ...

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Hi everyone! I'm trying to construct a protein ladder, which are markers used to indicate the molecular weight of proteins when running a SDS-PAGE. I have conjugated several different molecular weight proteins with dyes and ran them on ...

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"to be cloned" part is not needed. Sorry When making a gene construct (before inserting into an expression vector), do we have to place the desired gene, the reporter gene and the marker gene in any specific direction or orientation? I have seen in ...

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When making a gene construct to be cloned, do we have to place the desired gene, the reporter gene and the marker gene in any specific direction or orientation? I have seen in most cases the desired gene and the reporter gene have ...

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... we place the reporter gene under a different promoter? If not, what is the use of placing a selectable marker and a reporter gene under the same construct (without a fusion) ? Thank you Regards :)

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