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Construct

Construct

(noun) The combination of a bone graft, metal instrumentation, prosthetic devices and/or bone cement applied to a specific level of the spinal column in the setting of segmental spinal instability.


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blastn and phylogenetic tree

... lists for my unknown bacterial. There are 5 unknown bacteria that I need to identify. I would like to identify the bacteria using blastn and construct phylogenetic tree using neighbor joining. Can anyone help me how to do it, since I not sure and familiar with it. Please help me as fast as ...

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by juannajuann
Wed May 29, 2013 6:07 am
 
Forum: Molecular Biology
Topic: blastn and phylogenetic tree
Replies: 1
Views: 63

Protein ladder width increases down the gel?

Hi everyone! I'm trying to construct a protein ladder, which are markers used to indicate the molecular weight of proteins when running a SDS-PAGE. I have conjugated several different molecular weight proteins with dyes and ran them on ...

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by albert23
Wed Feb 27, 2013 12:55 am
 
Forum: General Discussion
Topic: Protein ladder width increases down the gel?
Replies: 2
Views: 658

Orientation of genes

"to be cloned" part is not needed. Sorry When making a gene construct (before inserting into an expression vector), do we have to place the desired gene, the reporter gene and the marker gene in any specific direction or orientation? I have seen in ...

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by Sue4
Mon Jan 28, 2013 9:03 pm
 
Forum: Molecular Biology
Topic: Orientation of genes
Replies: 2
Views: 537

Orientation of genes

When making a gene construct to be cloned, do we have to place the desired gene, the reporter gene and the marker gene in any specific direction or orientation? I have seen in most cases the desired gene and the reporter gene have ...

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by Sue4
Mon Jan 28, 2013 8:23 pm
 
Forum: Molecular Biology
Topic: Orientation of genes
Replies: 2
Views: 537

Reporter gene without fusion

... we place the reporter gene under a different promoter? If not, what is the use of placing a selectable marker and a reporter gene under the same construct (without a fusion) ? Thank you Regards :)

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by Sue4
Fri Jan 25, 2013 11:43 am
 
Forum: Molecular Biology
Topic: Reporter gene without fusion
Replies: 1
Views: 420
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