Dictionary » C » Constitutive expression

Constitutive expression

Definition

noun

Expression of a gene that is transcribed at a constant level.


Supplement

For example, the expression of housekeeping genes to produce proteins such as actin, GAPDH and ubiquitin.


See also: constitutive gene, gene expression


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Can you recommend a vector?

... the gene is under the control of the CMV promoter and we are getting constitutive silencing, this even happens when using a GFP reporter, where there is no phenotype. We can maintain expression (as detected by western and qPCR) of the transgene for 8-12 cell passages, ...

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by JimmyTee
Wed Jun 15, 2011 3:17 pm
 
Forum: Molecular Biology
Topic: Can you recommend a vector?
Replies: 0
Views: 1428

Re: Experimental evidence for evolution

... a gene, citT, encoding a citrate– succinate antiporter. High-level constitutive expression of this gene on a multicopy plasmid allows aerobic growth on citrate, but the native ...

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by robsabba
Wed Jun 23, 2010 2:25 pm
 
Forum: Evolution
Topic: Experimental evidence for evolution
Replies: 103
Views: 177631

Re: Experimental evidence for evolution

... a gene, citT, encoding a citrate– succinate antiporter. High-level constitutive expression of this gene on a multicopy plasmid allows aerobic growth on citrate, but the native ...

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by jevg
Tue Jun 22, 2010 9:58 pm
 
Forum: Evolution
Topic: Experimental evidence for evolution
Replies: 103
Views: 177631

Multiple genes cloning

... in tandem antibiotic resistance gene (ex. NPTII) under control of the constitutive promoter while the other two under the control of the inducible ... you spay them with the inducer (some chemical or hormone) to get the expression of the other 2 genes. Theoretically, you can put all three genes ...

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by Cat
Tue Jan 20, 2009 2:17 am
 
Forum: Molecular Biology
Topic: Multiple genes cloning
Replies: 7
Views: 5604

Please explain to me - CMVp

... for me or at least give me a hint. T7: Inducible promoter for bacterial expression (e.g. pET system), needs T7 polymerase which is not present in ... of a DE3 gene (can be or usually is controlled by the lac-operon) CMV: constitutive promoter for mammalian expression Do you have a shuttle vector ...

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by Duke
Tue Oct 02, 2007 11:37 am
 
Forum: Molecular Biology
Topic: Please explain to me - CMVp
Replies: 4
Views: 3630
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