Constitutive expression

... the gene is under the control of the CMV promoter and we are getting constitutive silencing, this even happens when using a GFP reporter, where there is no phenotype. We can maintain expression (as detected by western and qPCR) of the transgene for 8-12 cell passages, ...

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... a gene, citT, encoding a citrate– succinate antiporter. High-level constitutive expression of this gene on a multicopy plasmid allows aerobic growth on citrate, but the native ...

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... a gene, citT, encoding a citrate– succinate antiporter. High-level constitutive expression of this gene on a multicopy plasmid allows aerobic growth on citrate, but the native ...

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... in tandem antibiotic resistance gene (ex. NPTII) under control of the constitutive promoter while the other two under the control of the inducible ... you spay them with the inducer (some chemical or hormone) to get the expression of the other 2 genes. Theoretically, you can put all three genes ...

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... for me or at least give me a hint. T7: Inducible promoter for bacterial expression (e.g. pET system), needs T7 polymerase which is not present in ... of a DE3 gene (can be or usually is controlled by the lac-operon) CMV: constitutive promoter for mammalian expression Do you have a shuttle vector ...

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