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Conjugated protein

Conjugated protein

a protein that contains atleast one prosthetic group.


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Protein ladder width increases down the gel?

... the others. The ladder is basically combining 2ul of each of the 9 dye-conjugated proteins (~18ul total). The dye-conjugated protein that I loaded separately and combined in ...

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by albert23
Wed Feb 27, 2013 5:12 pm
 
Forum: General Discussion
Topic: Protein ladder width increases down the gel?
Replies: 2
Views: 1343

Protein ladder width increases down the gel?

Hi everyone! I'm trying to construct a protein ladder, which are markers used to indicate the molecular weight of proteins when running a SDS-PAGE. I have conjugated several different molecular weight proteins with dyes and ran them on a 12% gel. There ...

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by albert23
Wed Feb 27, 2013 12:55 am
 
Forum: General Discussion
Topic: Protein ladder width increases down the gel?
Replies: 2
Views: 1343

Re: Why is 1 absorbance of proteins ~ 1 mg/ml?

How much the protein absorbs light at 280nm will be a function of how much tryptophan and tyrosine are ... of interest at 260 nm. The absorbance of 280 nm light occurs because electrons in the conjugated pi orbitals of the aromatic amino acids can undergo energy-level transitions ...

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by jonmoulton
Thu Feb 17, 2011 4:43 pm
 
Forum: Molecular Biology
Topic: Why is 1 absorbance of proteins ~ 1 mg/ml?
Replies: 1
Views: 4279

Help with the design of an ELISA experiment

... the concentration of IgG in serum and what percentage of the cellular protein in said serum is a result of IgG. What I have to work with is mouse ... anti-mouse IgG (biotin and FC-HRP), goat anti rabbit IgG (biotin and ap-conjugated), goat anti-mouse IgG HRP (gamma chain), rabbit anti mouse serum. ...

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by bemardus
Tue Apr 27, 2010 10:39 pm
 
Forum: Molecular Biology
Topic: Help with the design of an ELISA experiment
Replies: 4
Views: 2963

BSA conjugation

... the sensitivity of the results in my DNA microarray chip. So far, I conjugated BSA to oligos (powder that was dissolved in water) and also conjugated BSA to SANH. Since oligos is relatively small and BSA is a large protein, I hoped to increase the sensitivity. After running a protein gel, ...

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by vsun
Mon Sep 28, 2009 1:14 am
 
Forum: Cell Biology
Topic: BSA conjugation
Replies: 0
Views: 935
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