Conjugated

But the buffer in the new ladder should be the same as the others. The ladder is basically combining 2ul of each of the 9 dye-conjugated proteins (~18ul total). The dye-conjugated protein that I loaded separately and combined in the ladder are from the same solution. In addition, I loaded ...

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... I'm trying to construct a protein ladder, which are markers used to indicate the molecular weight of proteins when running a SDS-PAGE. I have conjugated several different molecular weight proteins with dyes and ran them on a 12% gel. There is no problem with the size when I loaded each protein ...

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... you could specify the antibodies, for example: immmunihistochemistry using goat anti-VEGFR primary antibody and anti-goat-IgG secondary antibody conjugated to horseradish peroxidase.

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... where I place in VEGF sample and it wicks up and through a conjugate pad (made of fiber glass) that is saturated in 30 nm gold colloidal particle conjugated to anti-VEGF antibody. These should be moving together, and should bind to an aptamer that is immobilized on the nitrocellulose. The gold ...

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Hi Jack, I agree, though the xanthophylls might move a little. pi clouds might give some affinity with the conjugated mobile phase (benzene). - Jon

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