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Conjugated

Conjugated

Acting or operating as if joined, simultaneous.


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Protein ladder width increases down the gel?

But the buffer in the new ladder should be the same as the others. The ladder is basically combining 2ul of each of the 9 dye-conjugated proteins (~18ul total). The dye-conjugated protein that I loaded separately and combined in the ladder are from the same solution. In addition, I loaded ...

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by albert23
Wed Feb 27, 2013 5:12 pm
 
Forum: General Discussion
Topic: Protein ladder width increases down the gel?
Replies: 2
Views: 1717

Protein ladder width increases down the gel?

... I'm trying to construct a protein ladder, which are markers used to indicate the molecular weight of proteins when running a SDS-PAGE. I have conjugated several different molecular weight proteins with dyes and ran them on a 12% gel. There is no problem with the size when I loaded each protein ...

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by albert23
Wed Feb 27, 2013 12:55 am
 
Forum: General Discussion
Topic: Protein ladder width increases down the gel?
Replies: 2
Views: 1717

Re: Difference between ICC and IF

... you could specify the antibodies, for example: immmunihistochemistry using goat anti-VEGFR primary antibody and anti-goat-IgG secondary antibody conjugated to horseradish peroxidase.

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by jonmoulton
Mon Feb 25, 2013 5:11 pm
 
Forum: Molecular Biology
Topic: Difference between ICC and IF
Replies: 7
Views: 17137

Lateral Flow Assay

... where I place in VEGF sample and it wicks up and through a conjugate pad (made of fiber glass) that is saturated in 30 nm gold colloidal particle conjugated to anti-VEGF antibody. These should be moving together, and should bind to an aptamer that is immobilized on the nitrocellulose. The gold ...

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by knclayton
Wed Mar 07, 2012 11:03 pm
 
Forum: Cell Biology
Topic: Lateral Flow Assay
Replies: 1
Views: 1948

Re: thin layer chromatography

Hi Jack, I agree, though the xanthophylls might move a little. pi clouds might give some affinity with the conjugated mobile phase (benzene). - Jon

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by jonmoulton
Mon Nov 28, 2011 4:59 pm
 
Forum: Molecular Biology
Topic: thin layer chromatography
Replies: 4
Views: 2153
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