Conjugants

... I've done the transconjugant transformation of plasmid from E. coli to C. necator on LB plates without antibiotics. To isolate out the transconjugants, I transfer the bacteria colonies from LB plates to Simmon Citrate Agar plates which will select out the C.necator conjugants since E. coli ...

See entire post

... or acceptor than others). Of course you better have a non plasmid dependant way to select strain B from A if you want to be able to select for the conjugants. HTH Patrick

See entire post