Confluence

I was wondering if anyone could tell me the approximate number of cells I should be getting in my cell count just before cells are reaching confluence (70-80%). We take the number of cells counted with the cell counter and multiply by 15 mL because that is what it is suspended in.

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... with those I have obtained in the past. I must add that all of these experiments are carried out whilst the cells were at the same level of confluence (70-80%). In the past, culturing my cells for 2-4 days was sometimes necessary to obtain the desired confluence and this didn't negatively ...

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... from Pheonix cell and infect MEFs for 1st time. Afternoon: repeat 2nd infection about 6 hours after 1st infection Note: 1. MEFs are in 45~75% confluence during the 1st infection. 2. I use Polybrene at at a final concentration of 5ug/ml. 3. Some times, I use viral supernatant stock which is ...

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... wrong, in spite of what your teacher told you. Bacteriostatic or bactericidic do not change the result: once the colonies have grown in a lawn (to confluence), you won't see a difference if they die (they usually do not melt away, the walls are still there) or if they simply stop growing... or ...

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... be used (e.g., the human actin promoter). The engineered fibroblasts are then injected into the host, either alone or after having been grown to confluence on cytodex 3 microcarrier beads. The fibroblasts now produce the protein product. The fibroblasts can then be introduced into a patient as ...

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