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Confluence

Confluence

a flowing together; a joining of two or more streams.

Synonym: confluens.

Origin: L. Confluens


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question about G418 sceening test

... trying to determine a threshold concentration for G418 screening of stable transfected cells (Huh-7). I start with a T25 flask of Huh-7 about 80% confluence, and passed it to a 6 well plate, each is about 15% confluence. After 24hr incubation with 2 mL regular DMEM, I changed to DMEM with G418 ...

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by cavaliersun2003
Mon Nov 11, 2013 7:28 am
 
Forum: Cell Biology
Topic: question about G418 sceening test
Replies: 0
Views: 1085

C2C12 Confluence

I was wondering if anyone could tell me the approximate number of cells I should be getting in my cell count just before cells are reaching confluence (70-80%). We take the number of cells counted with the cell counter and multiply by 15 mL because that is what it is suspended in.

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by biolab
Mon Oct 15, 2012 5:11 pm
 
Forum: Cell Biology
Topic: C2C12 Confluence
Replies: 0
Views: 988

Cells hyper-sensitive to treatment after 48 hour culture

... with those I have obtained in the past. I must add that all of these experiments are carried out whilst the cells were at the same level of confluence (70-80%). In the past, culturing my cells for 2-4 days was sometimes necessary to obtain the desired confluence and this didn't negatively ...

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by danint
Thu Sep 20, 2012 2:20 pm
 
Forum: General Discussion
Topic: Cells hyper-sensitive to treatment after 48 hour culture
Replies: 0
Views: 963

problem with retroviral infection of MEFs

... from Pheonix cell and infect MEFs for 1st time. Afternoon: repeat 2nd infection about 6 hours after 1st infection Note: 1. MEFs are in 45~75% confluence during the 1st infection. 2. I use Polybrene at at a final concentration of 5ug/ml. 3. Some times, I use viral supernatant stock which is ...

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by wantz722
Tue Jul 08, 2008 4:36 am
 
Forum: Cell Biology
Topic: problem with retroviral infection of MEFs
Replies: 1
Views: 3752

Bacteria and disinfectants

... wrong, in spite of what your teacher told you. Bacteriostatic or bactericidic do not change the result: once the colonies have grown in a lawn (to confluence), you won't see a difference if they die (they usually do not melt away, the walls are still there) or if they simply stop growing... or ...

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by canalon
Mon Jun 16, 2008 1:44 pm
 
Forum: Microbiology
Topic: Bacteria and disinfectants
Replies: 2
Views: 15342
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