Conditions

... the primers sequence and it shows a specific sequence for the gene but until now I cannot solve this problem. I also tried to change thermocycling conditions but still the problem exists. Can anyone help me what to do?

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... rather than your stawman version. You are just ignoring my previous post and repeating yourself. Because they do not interbred under natural conditions, they are considered separate species. self contradiction you say they are different species but they can breed which means they are the ...

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... in its parents then that shows ns is wrong as ns is about traits already present i being passed on Because they do not interbred under natural conditions, they are considered separate species. self contradiction you say they are different species but they can breed which means they are the ...

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... a species is Let's look at lions and tigers. Lions and tigers do not interbred in nature, yet can be made to under artificial (human controlled) conditions. Because they do not interbred under natural conditions, they are considered separate species. This does leave us with a question, however. ...

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... with ratios and have an optimal ratio (1:1 with 1ug DNA and 1ug MAX transfection reagent). I have also tried putting the cells in hypothermic conditions which appears to have increased the expression by almost double, but this still isn't quite enough. Any other suggestions of something I ...

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