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Complementary strands

Complementary strands

(Science: molecular biology) two single strands of dna in which the nucleotide Sequence is such that they will bind as a result of base pairing throughout their full length.


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Re: mRNA Search for RT-PCR (U to T)

If we design primers to one strand out of two strands of cDNA then there is a chance that one of the primer will not be complementary to mRNA and it will not bind to mRNA.How can we synthesize cDNA from that mRNA?

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by microniranjan
Sat Oct 05, 2013 9:58 am
 
Forum: Molecular Biology
Topic: mRNA Search for RT-PCR (U to T)
Replies: 8
Views: 5165

DNA helix and gene

Yes and no, they do not. They are complementary to each other, so one gene sequence can be found on one strand and the second gene sequence on another (read in the opposite direction). However, both strands are required for transcription to mRNA since it's the complement strand that ...

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by Cat
Sun Aug 18, 2013 4:36 pm
 
Forum: Molecular Biology
Topic: DNA helix and gene
Replies: 2
Views: 2780

Re: parallel binding of miRNA with mRNA

... (parallel). I have never heard of a natural system making parallel complementary transcripts from opposite strands of an antiparallel DNA duplex. OK, there might be something new for us to learn about ...

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by jonmoulton
Thu Jan 17, 2013 4:39 pm
 
Forum: Genetics
Topic: parallel binding of miRNA with mRNA
Replies: 9
Views: 4739

parallel binding of miRNA with mRNA

if they are transcribed from opposite strands (on the same locus of course) than they are complementary and can bind to each other just as the DNA strands do

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by JackBean
Thu Jan 17, 2013 8:38 am
 
Forum: Genetics
Topic: parallel binding of miRNA with mRNA
Replies: 9
Views: 4739

Re: parallel binding of miRNA with mRNA

I have not heard of a case of parallel complementary binding. However, you said that the miRNA and mRNA are complementary and are transcribed from opposite strands of DNA. This makes miRNA and mRNA antiparallel because their templates, the two strands ...

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by jonmoulton
Wed Jan 16, 2013 3:32 pm
 
Forum: Genetics
Topic: parallel binding of miRNA with mRNA
Replies: 9
Views: 4739
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