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Complementary sequences

Complementary sequence

(Science: molecular biology) Nucleicacid base sequences that can form a double-stranded structure bymatching base pairs, the complementary sequence to G-T-a-C is C-a-T-G.


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Re: rna interference

... of the RNAi system. VIral RNA may be manufactured as two independent complementary strands by using the vial RNA-directed RNA polymerases. However, ... self-complementary region has been transcribed the two complementary sequences anneal together to form the hairpin. In the nucleus, the hairpin ...

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by jonmoulton
Mon Feb 09, 2009 5:17 pm
 
Forum: Molecular Biology
Topic: rna interference
Replies: 4
Views: 722

DNA microchip techniques

... each chip contains thousands of short, synthetic, single-stranded DNA sequences, which together add up to the normal gene in question. What is ... denatures the DNA in the samples - a process that separates the two complementary strands of DNA into single-stranded molecules. The next step ...

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by pguo
Mon Nov 24, 2008 6:44 pm
 
Forum: Molecular Biology
Topic: DNA microchip techniques
Replies: 0
Views: 453

Re: How to knock down a gene?

... or transcript expressed then interacts with the target mRNA by complementary base pairing (a sense-antisense interaction). The specific ... loaded into the RISC complex, which catalytically cleaves complementary sequences and inhibits translation of some mRNAs bearing partially-complementary ...

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by jonmoulton
Fri Oct 31, 2008 6:46 pm
 
Forum: Genetics
Topic: How to knock down a gene?
Replies: 1
Views: 806

Re: how to knock out a gene?

... the cytosol/nuclear compartment of cells. For (1), Morpholinos bind to complementary sequences in RNA and get in the way of other processes, like pre-mRNA splicing, mRNA translation ...

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by jonmoulton
Fri Oct 17, 2008 4:38 pm
 
Forum: Genetics
Topic: how to knock out a gene?
Replies: 4
Views: 1310

Re: splicing MO vs translation MO

... of the protein. I'd suggest you start with the coinjection (check the sequences of the oligos to see whether they are cross-complementary). If that doesn't get rid of the CE phenotype, consider using a coinjected p53 ...

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by jonmoulton
Tue Aug 19, 2008 4:22 pm
 
Forum: Molecular Biology
Topic: splicing MO vs translation MO
Replies: 14
Views: 2210
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