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Complementary sequences

Complementary sequence

(Science: molecular biology) Nucleicacid base sequences that can form a double-stranded structure bymatching base pairs, the complementary sequence to G-T-a-C is C-a-T-G.


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Re: Does complementary DNA include only coding sequences ?

Right - another technique, like RACE, is usually needed to extend the sequence across UTRs. If the primers are designed against genomic sequence, they can include UTR sequence, but are unlikely to capture the entire UTRs.

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by jonmoulton
Tue Oct 29, 2013 3:14 pm
 
Forum: Genetics
Topic: Does complementary DNA include only coding sequences ?
Replies: 4
Views: 1045

Re: Does complementary DNA include only coding sequences ?

cDNA can contain 5' and/or 3' UTR sequence but not introns.

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by jonmoulton
Mon Oct 28, 2013 3:46 pm
 
Forum: Genetics
Topic: Does complementary DNA include only coding sequences ?
Replies: 4
Views: 1045

Does complementary DNA include only coding sequences ?

Hello everyone, I was wondering if complementary DNA is a copy of the entire messenger RNA or only a copy of the coding parts ? I'm really confused... For example, if I have the mRNA of a particular gene with some non-coding parts, will the ...

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by ophelia15
Sat Oct 19, 2013 10:25 pm
 
Forum: Genetics
Topic: Does complementary DNA include only coding sequences ?
Replies: 4
Views: 1045

Population Genetics Questions

... love some guidance. Thanks in advance. 1. You obtain the following sequences from different genomic clones from one region of the human genome. ... so you will need to figure out which one it is and use its reverse complementary strand. Sequence 1: AACAAAAATGTTGTTATTTTTATTTCAGATG Sequence ...

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by animus31
Wed Nov 21, 2012 3:36 pm
 
Forum: Genetics
Topic: Population Genetics Questions
Replies: 3
Views: 2013

RNA as genetic material

... less stable than DNA because it is more prone to hydrolysis. Third, the complementary base to adenine is not thymine, as it is in DNA, but rather ... rRNA, snRNAs, and other non-coding RNAs, contain self-complementary sequences that allow parts of the RNA to fold and pair with itself to form ...

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by bellyjelly
Tue Jul 12, 2011 7:41 am
 
Forum: Molecular Biology
Topic: RNA as genetic material
Replies: 9
Views: 19257
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