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Dictionary » C » Complementary sequences Complementary sequences(Science: molecular biology) Nucleicacid base sequences that can form a double-stranded structure bymatching base pairs, the complementary sequence to G-T-a-C is C-a-T-G. ![]()
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Results from our forumRe: rna interference... of the RNAi system. VIral RNA may be manufactured as two independent complementary strands by using the vial RNA-directed RNA polymerases. However, ... self-complementary region has been transcribed the two complementary sequences anneal together to form the hairpin. In the nucleus, the hairpin ...
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DNA microchip techniques... each chip contains thousands of short, synthetic, single-stranded DNA sequences, which together add up to the normal gene in question. What is ... denatures the DNA in the samples - a process that separates the two complementary strands of DNA into single-stranded molecules. The next step ...
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Re: How to knock down a gene?... or transcript expressed then interacts with the target mRNA by complementary base pairing (a sense-antisense interaction). The specific ... loaded into the RISC complex, which catalytically cleaves complementary sequences and inhibits translation of some mRNAs bearing partially-complementary ...
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Re: how to knock out a gene?... the cytosol/nuclear compartment of cells. For (1), Morpholinos bind to complementary sequences in RNA and get in the way of other processes, like pre-mRNA splicing, mRNA translation ...
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Re: splicing MO vs translation MO... of the protein. I'd suggest you start with the coinjection (check the sequences of the oligos to see whether they are cross-complementary). If that doesn't get rid of the CE phenotype, consider using a coinjected p53 ...
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