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Complementary

Complementary

Supplying a defect or helping to do so, making complete, accessory.

Origin: L. Complere = to fill


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Re: Any ideas for vaccine development or anti-viral drugs???

... PMOs represent a promising class of therapeutic agents to combat filoviral infections. Previously we have shown that mice treated with a PMO complementary to a region spanning the start codon of VP24 mRNA were protected against lethal Ebolavirus challenge. In the present study, we report ...

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by jonmoulton
Mon Sep 15, 2014 3:04 pm
 
Forum: Cell Biology
Topic: Any ideas for vaccine development or anti-viral drugs???
Replies: 2
Views: 479

Re: Does complementary DNA include only coding sequences ?

Right - another technique, like RACE, is usually needed to extend the sequence across UTRs. If the primers are designed against genomic sequence, they can include UTR sequence, but are unlikely to capture the entire UTRs.

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by jonmoulton
Tue Oct 29, 2013 3:14 pm
 
Forum: Genetics
Topic: Does complementary DNA include only coding sequences ?
Replies: 4
Views: 2031

Re: Does complementary DNA include only coding sequences ?

cDNA can contain 5' and/or 3' UTR sequence but not introns.

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by jonmoulton
Mon Oct 28, 2013 3:46 pm
 
Forum: Genetics
Topic: Does complementary DNA include only coding sequences ?
Replies: 4
Views: 2031

Does complementary DNA include only coding sequences ?

Hello everyone, I was wondering if complementary DNA is a copy of the entire messenger RNA or only a copy of the coding parts ? I'm really confused... For example, if I have the mRNA of a particular gene with some non-coding parts, will the ...

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by ophelia15
Sat Oct 19, 2013 10:25 pm
 
Forum: Genetics
Topic: Does complementary DNA include only coding sequences ?
Replies: 4
Views: 2031

Re: mRNA Search for RT-PCR (U to T)

If we design primers to one strand out of two strands of cDNA then there is a chance that one of the primer will not be complementary to mRNA and it will not bind to mRNA.How can we synthesize cDNA from that mRNA?

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by microniranjan
Sat Oct 05, 2013 9:58 am
 
Forum: Molecular Biology
Topic: mRNA Search for RT-PCR (U to T)
Replies: 8
Views: 5345
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