Competitive binding assay
This assay is used to measure the concentration of biologically specific receptors in a sample. One example of its use it to measure antibodies in a sample by radioimmunoassay wherein a radioactively labeled antigens are introduced to bind to specific antibodies. The radioactivity in the products is then compared with the standard reaction to quantify antibodies.
Results from our forum
... protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254 Chayen A, Avron ... acid in 50 mM acetate buffer, pH 5.5. In the assay using GM1 and GD1, the buffer contained 0.1% ... against Mu-Neu5Ac as substrate revealed competitive patterns (Fig. 3) for both Neu5Ac2en ...
See entire post