Dictionary » C » Competitive binding assay

Competitive binding assay

Definition

An assay based on the competition between labeled and unlabeled ligand for the reactive sites of a particular substance


Supplement

This assay is used to measure the concentration of biologically specific receptors in a sample. One example of its use it to measure antibodies in a sample by radioimmunoassay wherein a radioactively labeled antigens are introduced to bind to specific antibodies. The radioactivity in the products is then compared with the standard reaction to quantify antibodies.

The use of this assay extends to measuring other compounds that can be coupled with antigens, such as drugs, enzymes, and hormones.


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The Fiber Disease

... protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254 Chayen A, Avron ... acid in 50 mM acetate buffer, pH 5.5. In the assay using GM1 and GD1, the buffer contained 0.1% ... against Mu-Neu5Ac as substrate revealed competitive patterns (Fig. 3) for both Neu5Ac2en ...

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by Nadas Moksha
Sun Dec 10, 2006 3:16 am
 
Forum: Human Biology
Topic: The Fiber Disease
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