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Competent cell

Competent cell

(Science: cell biology) cells that are able to take up exogenous genetic material.


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Problems making chemically competent cells

... quite a bit so I apologise in advance! I am looking to trouble shoot my competent cell making process. I make competent cells for a number of labs, but struggle to get the transformation ...

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by lilruthc
Fri Feb 08, 2013 1:09 pm
 
Forum: Molecular Biology
Topic: Problems making chemically competent cells
Replies: 1
Views: 1839

Competent cell growth on all my plates (multi resistant)

I have been given a batch of XL1 blue cells. I made them competent using calcium chloride procedure. To test them, i plated them out on agar plate containing: ...

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by emadsadeghian
Fri Jan 04, 2013 12:27 pm
 
Forum: Molecular Biology
Topic: Competent cell growth on all my plates (multi resistant)
Replies: 0
Views: 1242

molecular cloning failure

... I am doing my master thesis in the cloning of keratinase sequence in competent cell.I tried several different methods and procedure for the cloning and I got the colonies ...

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by siddharthsameer
Tue Oct 23, 2012 6:33 pm
 
Forum: Molecular Biology
Topic: molecular cloning failure
Replies: 3
Views: 1212

bacterial culture growth

... the ph of Preculture.I made a a preculture of e.coli tof 10 for the competent cell, and i was asked to measure the ph of the preculture, and I had 3 precultre in different ...

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by siddharthsameer
Wed Jul 25, 2012 8:49 am
 
Forum: Cell Biology
Topic: bacterial culture growth
Replies: 1
Views: 1598

Re: restriction analysis

... with the plasmid and then i have to to subclone it into E.coli TOF competent cell and then i have to plate it select for the colonies and then do the mini prep to get the ...

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by siddharthsameer
Mon Jul 16, 2012 12:33 pm
 
Forum: Molecular Biology
Topic: restriction analysis
Replies: 14
Views: 5890
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