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Competency

Competency

(Science: cell biology) An ephemeral state, induced by treatment with cold cations, during which bacterial cells are capable of uptaking foreign dna.


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Making Competent Cell

I must say that I never measured the competency level, and in most case got my transformants (as for most of the other cases, that is common when you try to express a protein that is toxic for your bacteria). I have been known to "steal" ...

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by canalon
Thu Sep 08, 2011 5:07 pm
 
Forum: Microbiology
Topic: Making Competent Cell
Replies: 5
Views: 3831

Making Competent Cell

The competency level of the cell don't always hit 1x10exp8 to 1x10exp9 cfu's/ug. Sometimes is just too low, result are not always consistent. Is there anything to take note? I know that TFb1 and TFb2 need to dissolve individually ...

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by kenpier
Thu Sep 08, 2011 3:18 am
 
Forum: Microbiology
Topic: Making Competent Cell
Replies: 5
Views: 3831

Re: dialogue for short film

... including a capsid surrounding it. Therefore, if you are producing virions, there are no naked DNA to be used for transformation so maybe cell competency should not be mentioned. Did I make any sense? guys? lots of luck.... wish I could be a scriptwriter, ha ha peace.

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by mkwaje
Sat Jan 30, 2010 10:41 am
 
Forum: Microbiology
Topic: dialogue for short film
Replies: 4
Views: 4066

Natural selection is proven wrong

... mind that finds its rudiments in atheistic philosophy--not Judeo-Christian or Islamic faith. The government has a certain obligation to ensure the competency of public education, which has a certain obligation to ensure that what our children are taught bears at least a passing resemblance to reality, ...

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by AFJ
Sun May 03, 2009 10:37 pm
 
Forum: Evolution
Topic: Natural selection is proven wrong
Replies: 247
Views: 649580

Natural selection is proven wrong

... who has legislated anything but these teachings, and philosophy out of our education system. The government has a certain obligation to ensure the competency of public education, which has a certain obligation to ensure that what our children are taught bears at least a passing resemblance to reality, ...

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by alextemplet
Sun May 03, 2009 7:59 pm
 
Forum: Evolution
Topic: Natural selection is proven wrong
Replies: 247
Views: 649580
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