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Column chromatography

Definition

noun

A type of chromatography wherein the stationary bed is within a tube (packed column)


Supplement

Based on the chromatographic bed shape, there are two major forms of chromatography: the planar chromatography and the column chromatography.


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Scientific paper on DNA replication & gel electrophoresis

... replication fork. This was the bulk reaction. -Then used gel-filtration chromatography to remove all unbound proteins and then readded only the 3 ... using gel electrophoresis from before was preformed. This was the column isolated reaction. Gel migrations from the bulk reaction and column-isolated ...

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by dekk1525
Sat Sep 08, 2012 11:17 pm
 
Forum: Molecular Biology
Topic: Scientific paper on DNA replication & gel electrophoresis
Replies: 0
Views: 4923

Why dialyse DMEM before protein purification (Ni-Affinity)?

... Ni-NTA Agarose was added and finally these beads where put on a column. Some washing procedures followed (his-dialysis-buffer with either ... DMEM that would make it difficult (impossible?) to run the ni-affinity chromatography? Would the protein precipitate? If yes, why then? I've been ...

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by LittleBird
Sun May 20, 2012 3:43 pm
 
Forum: Molecular Biology
Topic: Why dialyse DMEM before protein purification (Ni-Affinity)?
Replies: 5
Views: 2782

pI's of 2 proteins

... ratio of basic to acidic residues on the molecule. A cation-exchange column, specifically carboxymethyl (CMC) is used to help purify cytochrome c. This is because the pH for chromatography is below the pI of cytochrome c, thus leaving the molecule with an overall ...

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by mataiquang
Wed Mar 23, 2011 10:53 pm
 
Forum: General Discussion
Topic: pI's of 2 proteins
Replies: 1
Views: 2614

chromatography - CIP

... because the extract is really dirty (just yeasterday, the Octyl Sep was totally black :lol:, now it looks a little better...), I need to clean the columns often and efficiently. So, I was looking to manuals, how to do that, but I have still two question (more like why, insted of how) 1)in Octyl ...

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by JackBean
Thu Feb 25, 2010 7:58 am
 
Forum: Molecular Biology
Topic: chromatography - CIP
Replies: 0
Views: 1213

G genes

... Recombinant His-tagged proteins (and how they are purified by affinity chromatography on a nickel-agarose column) Thank you very much for your help! - Tim

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by zhetimxu
Sun Jun 28, 2009 7:21 pm
 
Forum: General Discussion
Topic: G genes
Replies: 1
Views: 2012
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